Human ErbB2/Her2 DuoSet ELISA

Name: Human ErbB2/Her2 DuoSet ELISA
Brand: R&D Systems
SKU: DY1129B
Rating: 5 (1 reviews)

Catalog # DY1129B | R&D Systems, Inc. a Bio-Techne Brand

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Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	54.7 - 3,500 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human ErbB2/Her2 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human ErbB2/Her2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ErbB2. The suggested diluent is suitable for the analysis of most cell culture supernate, serum, plasma, urine, saliva, and human milk samples. Diluents for complex matrices, such as serum plasma, urine, saliva, and human milk should be evaluated prior to use in this DuoSet.

Product Specifications for Human ErbB2/Her2 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human ErbB2/Her2 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human ErbB2/Her2 DuoSet ELISA

	Human ErbB2 / Her2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.

Item	Value
Long Name	Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs	2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names	CD340, CD340 antigen, EC 2.7.10, EGFR2, ErbB2, HER-2, HER2, HER2EC 2.7.10.1, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEUHER-2/neu, NGL, NGLTKR1, Neu Oncogene, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, herstatin, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, receptor tyrosine-protein kinase erbB-2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Citations for Human ErbB2/Her2 DuoSet ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human ErbB2/Her2 DuoSet ELISA include:

Species: Human
Sample Types: Serum

L Gutiérrez- et al. (2023-03-05), MoS2-Carbon Nanodots as a New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection Biosensors, 2023-013(3).
PMID: 36979560

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Human ErbB2/Her2 DuoSet ELISA

Name: Anonymous

Sample Tested: CHO Chinese hamster ovary cell line

Verified Customer | Posted 08/03/2017

Using this ELISA kit to test the ErbB2 level of CHO cell at different conditions, very sensitive and easy to use.

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Product Documents for Human ErbB2/Her2 DuoSet ELISA

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Human ErbB2/Her2 DuoSet ELISA

Catalog # DY1129B | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Summary for Human ErbB2/Her2 DuoSet ELISA

Product Specifications for Human ErbB2/Her2 DuoSet ELISA

Kit Contents for Human ErbB2/Her2 DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human ErbB2/Her2 DuoSet ELISA

Preparation and Storage

Background: ErbB2/Her2

Citations for Human ErbB2/Her2 DuoSet ELISA (1)

Customer Reviews for Human ErbB2/Her2 DuoSet ELISA (1)

Human ErbB2/Her2 DuoSet ELISA

Product Documents for Human ErbB2/Her2 DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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