Human Total ErbB2/Her2 DuoSet IC ELISA

Name: Human Total ErbB2/Her2 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC1129-2
Price: 440 USD

Catalog # DYC1129-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell Lysates
Human Total ErbB2/Her2 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total ErbB2/Her2 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total ErbB2 / Her2 in cell lysates. An immobilized capture antibody specific for ErbB2 / Her2 binds both phosphorylated and unphosphorylated ErbB2 / Her2. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total ErbB2/Her2 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total ErbB2/Her2 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total ErbB2/Her2 DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.

Item	Value
Long Name	Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs	2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names	CD340, CD340 antigen, EC 2.7.10, EGFR2, ErbB2, HER-2, HER2, HER2EC 2.7.10.1, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEUHER-2/neu, NGL, NGLTKR1, Neu Oncogene, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, herstatin, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, receptor tyrosine-protein kinase erbB-2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Citations for Human Total ErbB2/Her2 DuoSet IC ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total ErbB2/Her2 DuoSet IC ELISA include:

Species: Human
Sample Types: Cells

KK Kim et al. (2020-02-24), Development of Potent Forchlorfenuron Analogs and Their Cytotoxic Effect in Cancer Cell Lines Sci Rep, 2020-010(1):3241.
PMID: 32094384
Species: Human
Sample Types: Tissue Homogenates

Yamashita-Kashima Y et al. (2011-06-23), Pertuzumab in combination with trastuzumab shows significantly enhanced antitumor activity in HER2-positive human gastric cancer xenograft models. Clin. Cancer Res., 2011-017(15):5060-70.
PMID: 21700765
Species: Human
Sample Types: Cell Lysates

Hickinson DM et al. (2009-06-01), Identification of biomarkers in human head and neck tumor cell lines that predict for in vitro sensitivity to gefitinib. Clin Transl Sci, 2009-02(3):183-92.
PMID: 20443891

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