Human ErbB3/Her3 DuoSet ELISA

Name: Human ErbB3/Her3 DuoSet ELISA
Brand: R&D Systems
SKU: DY348
Price: 745 USD

Catalog # DY348 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human ErbB3/Her3 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human ErbB3/Her3 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ErbB3. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human ErbB3/Her3 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human ErbB3/Her3 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human ErbB3/Her3 DuoSet ELISA

	Human ErbB3 / Her3 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 binds neuregulins NRG1 and NRG2, but lacks a working kinase domain; heterodimerization with another ErbB receptor is required for signaling. ErbB3 is important in development of Schwann cells, neural crest cells, heart valves and the sympathetic nervous system. When expressed with ErbB2 in breast, skin, ovary and gastrointestinal tumors, ErbB3 is a potent mitogen.

Item	Value
Long Name	Receptor Tyrosine Protein Kinase ErbB3
Entrez Gene IDs	2065 (Human); 13867 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, ErbB-3, ErbB3, HER3, HER3c-erbB-3, LCCS2, MDA-BF-1, MGC88033, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, c-erbB3, erbB3-S, lethal congenital contracture syndrome 2, p180-ErbB3, p45-sErbB3, p85-sErbB3, receptor tyrosine-protein kinase erbB-3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Citations for Human ErbB3/Her3 DuoSet ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human ErbB3/Her3 DuoSet ELISA include:

Species: Human
Sample Types: Tissue Homogenates

D Gutsch et al. (2021-01-29), Inhibition of HER Receptors Reveals Distinct Mechanisms of Compensatory Upregulation of Other HER Family Members: Basis for Acquired Resistance and for Combination Therapy Cells, 2021-010(2).
PMID: 33572976
Species: Human
Sample Types: Serum

(2016-09-15), Patritumab plus trastuzumab and paclitaxel in human epidermal growth factor receptor 2-overexpressing metastatic breast cancer Cancer Sci., 2016-0107(10):1465-1470.
PMID: 27452985
Species: Human
Sample Types: Tissue Homogenates

(2016-08-11), Heregulin-1ï¿½ and HER3 in hepatocellular carcinoma: status and regulation by insulin J Exp Clin Cancer Res, 2016-035(1):126.
PMID: 27514687
Species: Human
Sample Types: Peptide

Lin SH et al. (2008-06-15), Soluble ErbB3 levels in bone marrow and plasma of men with prostate cancer. Clin. Cancer Res., 2008-014(12):3729-36.
PMID: 18559590

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Showing 1 - 4 of 4 citations Showing All

Product Documents for Human ErbB3/Her3 DuoSet ELISA

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