Human Total HGFR/c-MET DuoSet IC ELISA

Name: Human Total HGFR/c-MET DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC358-2
Price: 440 USD

Catalog # DYC358-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	125 - 8,000 pg/mL
Sample Types:	Cell Lysates
Human Total HGFR/c-MET DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total HGFR/c-MET DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total HGF R / c-MET in cell lysates. An immobilized capture antibody specific for HGF R / c-MET binds both phosphorylated and unphosphorylated HGF R / c-MET. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total HGFR/c-MET DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total HGFR/c-MET DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total HGFR/c-MET DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGFR/c-MET

HGF receptor, a product of the proto-oncogene c-met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. c-MET is synthesized as a single-chain precursor (pr170) which undergoes post-translational glycosylation and proteolytic cleavage to give rise to the heterodimeric mature form.

Item	Value
Long Name	Hepatocyte Growth Factor Receptor
Entrez Gene IDs	4233 (Human); 17295 (Mouse)
Alternate Names	AUTS9, EC 2.7.10, EC 2.7.10.1, HGF R, HGF receptor, HGF/SF receptor, HGFR, MET, Met (c-Met), Proto-oncogene c-Met, RCCP2, SF receptor, Scatter factor receptor, Tyrosine-protein kinase Met, c-MET, cMET, hepatocyte growth factor receptor, met proto-oncogene (hepatocyte growth factor receptor), met proto-oncogene tyrosine kinase, oncogene MET

Citations for Human Total HGFR/c-MET DuoSet IC ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total HGFR/c-MET DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

A Amaro et al. (2020-06-04), Potential Onco-Suppressive Role of miR122 and miR144 in Uveal Melanoma through ADAM10 and C-Met Inhibition Cancers (Basel), 2020-012(6).
PMID: 32512881
Species: Human
Sample Types: Tissue Homogenates

Oh et al. (2014-05-15), USP8 modulates ubiquitination of LRIG1 for Met degradation. Sci Rep, 2014-04(0):4980.
PMID: 24828152
Species: Human
Sample Types: Cell Culture Supernates

Fu et al. (2013-03-01), Shedding of c-Met ectodomain correlates with c-Met expression in non-small cell lung cancer. Biomarkers, 2013-018(2):126-35.
PMID: 23410046
Species: Human
Sample Types: Cell Lysates

Xu L et al. (2010-02-15), Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells. Oncogene, 2010-029(18):2616-27.
PMID: 20154724

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