Human Total EphB4 DuoSet IC ELISA

Name: Human Total EphB4 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC3038-2

Catalog # DYC3038-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

Discontinued Product

DYC3038-2 has been discontinued. View all EphB4 products.

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell Lysates
Human Total EphB4 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Technical Data
Citations (1) / Reviews

Summary for Human Total EphB4 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total EphB4 in cell lysates. An immobilized capture antibody specific for EphB4 binds both phosphorylated and unphosphorylated EphB4. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total EphB4 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total EphB4 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EphB4

Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-B ligands are referred to as EphB. All Eph receptors contain an N-terminal Ig-like domain, a cysteine-rich region with 19 conserved cysteines and two fibronectin type III domains. The cytoplasmic region contains a typical tyrosine kinase organization.

Item	Value
Long Name	Eph Receptor B4
Entrez Gene IDs	2050 (Human); 13846 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, HTKephrin receptor EphB4, Htk, Mdk2, Myk1, Tyro11, Tyrosine-protein kinase TYRO11, Tyrosine-protein kinase receptor HTK, ephrin type-B receptor 4, hepatoma transmembrane kinase, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3

Citations for Human Total EphB4 DuoSet IC ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total EphB4 DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

Pennisi A et al. (2009-07-13), The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth. Blood, 2009-0114(9):1803-12.
PMID: 19597185

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Product Documents for Human Total EphB4 DuoSet IC ELISA

Product Datasheets

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