Human Total Flt-3/Flk-2 DuoSet IC ELISA

Name: Human Total Flt-3/Flk-2 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC912-2
Price: 440 USD

Catalog # DYC912-2 | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell Lysates
Human Total Flt-3/Flk-2 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total Flt-3/Flk-2 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Flt-3 / Flk-2 in cell lysates. An immobilized capture antibody specific for Flt-3 / Flk-2 binds both phosphorylated and unphosphorylated Flt-3 / Flk-2. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total Flt-3/Flk-2 DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and STK-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes c-kit, the receptor for SCF, and fms, the receptor for M-CSF. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin.

Item	Value
Long Name	fms-like Tyrosine Kinase 3
Entrez Gene IDs	2322 (Human); 14255 (Mouse)
Alternate Names	CD135, CD135 antigen, EC 2.7.10, FL cytokine receptor, FLK2, FLT3 receptor tyrosine kinase, Flk-2, Flt-3, Flt3, Fms-like tyrosine kinase 3, STK-1, STK1EC 2.7.10.1, Stem cell tyrosine kinase 1, Tyrosine-protein kinase receptor FLT3, fetal liver kinase 2, fms-related tyrosine kinase 3, growth factor receptor tyrosine kinase type III

Citations for Human Total Flt-3/Flk-2 DuoSet IC ELISA

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Human Total Flt-3/Flk-2 DuoSet IC ELISA

Catalog # DYC912-2 | R&D Systems, Inc. a Bio-Techne Brand

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Summary for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Product Specifications for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Kit Contents for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Other Reagents Required

Scientific Data Examples for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Preparation and Storage

Background: Flt-3/Flk-2

Citations for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Product Documents for Human Total Flt-3/Flk-2 DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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