Which phosphorylated sites are recognized by this assay?

Name: Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC4170-2
Price: 440 USD

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Catalog # DYC4170-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Sample Types:	Cell Lysates
Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15-(96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Summary for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human VEGF R1 / Flt-1 in cell lysates. An immobilized capture antibody specific for VEGF R1 / Flt-1 binds both phosphorylated and unphosphorylated VEGF R1 / Flt-1. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Item	Value
Long Name	Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs	2321 (Human); 14254 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, FLT, FLT1, FRT, Flt-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF R1, VEGFR-1, VEGFR1, Vascular permeability factor receptor, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor), vascular endothelial growth factor receptor 1

Citations for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

T Yao et al. (2022-06-08), Thiol-ene conjugation of VEGF peptide to electrospun scaffolds as potential application for angiogenesis Bioactive materials, 2022-020(0):306-317.
PMID: 35755423
Species: Human
Sample Types: Nuclear Extract

JAF Silva et al. (2021-08-30), Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells Scientific Reports, 2021-011(1):17400.
PMID: 34462507
Species: Human
Sample Types: Cell Culture Supernates

M Su et al. (2017-11-23), Andrographolide enhanced 5-fluorouracil-induced antitumor effect in colorectal cancer via inhibition of c-MET pathway Drug Des Devel Ther, 2017-111(0):3333-3341.
PMID: 29200829
Species: Bovine
Sample Types: Cell Lysates

Cai J et al. (2011-10-20), gamma-Secretase and presenilin mediate cleavage and phosphorylation of vascular endothelial growth factor receptor-1. J. Biol. Chem., 2011-1286(49):42514-23.
PMID: 22016384

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FAQs for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Which phosphorylated sites are recognized by this assay?

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Catalog # DYC4170-2 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Summary for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Product Specifications for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Kit Contents for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Other Reagents Required

Preparation and Storage

Background: VEGFR1/Flt-1

Citations for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA (4)

FAQs for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Product Documents for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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