Human M-CSF R/CD115 DuoSet ELISA

Name: Human M-CSF R/CD115 DuoSet ELISA
Brand: R&D Systems
SKU: DY329
Price: 745 USD

Catalog # DY329 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human M-CSF R/CD115 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human M-CSF R/CD115 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human M-CSF R. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human M-CSF R/CD115 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human M-CSF R/CD115 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

Scientific Data Examples for Human M-CSF R/CD115 DuoSet ELISA

	Human M-CSF R / CD115 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF R/CD115

M-CSF R/CD115, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes c-kit, the receptor for SCF, and the alpha and beta receptors for PDGF. M-CSF R/CD115 is expressed primarily on cells of the monocyte/macrophage lineage and in various tissues of the developing placenta.

Item	Value
Long Name	Macrophage Colony Stimulating Factor Receptor
Entrez Gene IDs	1436 (Human); 12978 (Mouse)
Alternate Names	CD115, CD115 antigen, CSF-1-R, CSF1R, CSFR, EC 2.7.10.1, FMS proto-oncogene, FMSFIM2, M-CSF R, M-CSFR, MCSFR, McDonough feline sarcoma viral (v-fms) oncogene homolog, Proto-oncogene c-Fms, c-fms, colony stimulating factor 1 receptor, macrophage colony stimulating factor I receptor, macrophage colony-stimulating factor 1 receptor

Citations for Human M-CSF R/CD115 DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human M-CSF R/CD115 DuoSet ELISA include:

Species: Human
Sample Types: Cell Lysates

M Banchi et al. (2020-07-01), Synergistic activity of linifanib and irinotecan increases the survival of mice bearing orthotopically implanted human anaplastic thyroid cancer Am J Cancer Res, 2020-010(7):2120-2127.
PMID: 32775005
Species: Human
Sample Types: Cell Lysates

SJ Wang et al. (2020-05-22), Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy Sci Adv, 2020-06(21):eaaz8521.
PMID: 32494745
Species: Human
Sample Types: Cell Culture Supernates

James DE et al. (2010-06-11), The relative timing of exposure to phagocytosable particulates and to osteoclastogenic cytokines is critically important in the determination of myeloid cell fate. J. Immunol., 2010-0185(2):1265-73.
PMID: 20543106

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Product Documents for Human M-CSF R/CD115 DuoSet ELISA

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