Human Total VEGF R3/Flt-4 DuoSet IC ELISA

Name: Human Total VEGF R3/Flt-4 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC3491-2

Catalog # DYC3491-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

Discontinued Product

DYC3491-2 has been discontinued. View all VEGFR3/Flt-4 products.

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell Lysates
Human Total VEGF R3/Flt-4 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Total VEGF R3/Flt-4 DuoSet IC ELISA

Technical Data
Citations (1) / Reviews

Summary for Human Total VEGF R3/Flt-4 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total VEGF R3 / Flt-4 in cell lysates. An immobilized capture antibody specific for VEGF R3 / Flt-4 binds both phosphorylated and unphosphorylated VEGF R3 / Flt-4. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total VEGF R3/Flt-4 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total VEGF R3/Flt-4 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR3/Flt-4

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Item	Value
Long Name	Vascular Endothelial Growth Factor Receptor 3
Entrez Gene IDs	2324 (Human); 14257 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, FLT4, Flt-4, LMPH1A, PCLFLT41, Tyrosine-protein kinase receptor FLT4, VEGF R3, VEGFR-3, VEGFR3, VEGFR3Fms-like tyrosine kinase 4, fms-related tyrosine kinase 4, soluble VEGFR3 variant 1, soluble VEGFR3 variant 2, soluble VEGFR3 variant 3, vascular endothelial growth factor receptor 3

Citations for Human Total VEGF R3/Flt-4 DuoSet IC ELISA (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Total VEGF R3/Flt-4 DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

Harris et al. (2013-02-12), The propeptides of VEGF-D determine heparin binding, receptor heterodimerization, and effects on tumor biology. J Biol Chem, 2013-0288(12):8176-86.
PMID: 23404505

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Product Documents for Human Total VEGF R3/Flt-4 DuoSet IC ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS