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Human VEGFR1/Flt-1 Quantikine ELISA Kit Best Seller

Catalog # DVR100C | R&D Systems, Inc. a Bio-Techne Brand
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DVR100C
PDVR100C
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Cell Lysates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (100 µL)

Sensitivity

8.46 pg/mL

Assay Range

31.3-2000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Product Summary for Human VEGFR1/Flt-1 Quantikine ELISA Kit

The Quantikine® Human VEGF R1/Flt-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF R1 in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF R1.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human VEGF R1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Sample TypeMean (pg/mLRange (pg/mL)
Serum (n=30)385294-478
EDTA Plasma (n=30)292208-391
Heparin Plasma (n=30)279216-377
Saliva (n=10)944346-1827

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 207 610 1207 207 616 1205
Standard Deviation 5.16 14.9 35.6 13.9 38.9 74.0
CV% 2.5 2.4 2.9 6.7 6.3 6.1

Recovery for Human VEGFR1/Flt-1 Quantikine ELISA Kit

The recovery of human VEGF R1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 87-110
Cell Lysis Buffer (n=4) 94 86-108
EDTA Plasma (n=4) 92 84-98
Heparin Plasma (n=4) 89 81-96
Saliva (n=4) 109 96-124
Serum (n=4) 104 93-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human VEGF R1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human VEGF R1/Flt-1 ELISA Linearity
Human VEGF R1/Flt-1 ELISA Linearity

Scientific Data Images for Human VEGFR1/Flt-1 Quantikine ELISA Kit

Human VEGF R1/Flt-1 Standard Curve

Human VEGF R1/Flt-1 Standard Curve

Human VEGF R1 Quantikine ELISA Kit Linearity Competitor Comparison

Human VEGF R1 Quantikine ELISA Kit Linearity Competitor Comparison

Human VEGF R1 Quantikine ELISA Kit Sensitivity Competitor Comparison

Human VEGF R1 Quantikine ELISA Kit Sensitivity Competitor Comparison

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Gene Symbol

FLT1

Product Documents for Human VEGFR1/Flt-1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VEGFR1/Flt-1 Quantikine ELISA Kit

For research use only

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  • Human VEGFR1/Flt-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Verified Customer | Posted 12/11/2021
    Human VEGFR1/Flt-1 Quantikine ELISA Kit DVR100C
  • Human VEGFR1/Flt-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Verified Customer | Posted 08/26/2020

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