Human Total Insulin R DuoSet IC ELISA

Name: Human Total Insulin R DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC1544-2
Price: 440 USD

Catalog # DYC1544-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	1250 - 80,000 pg/mL
Sample Types:	Cell Lysates
Human Total Insulin R DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total Insulin R DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Insulin R / CD220 in cell lysates. An immobilized capture antibody specific for Insulin R / CD220 binds both phosphorylated and unphosphorylated Insulin R / CD220. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total Insulin R DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total Insulin R DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total Insulin R DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Insulin R/CD220

Insulin Receptor (INSR, CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases. The mature receptor contains an extracellular alpha subunit and an extracellular/transmembrane/cytoplasmic beta subunit. Alternative splicing generates A and B isoforms which may homodimerize or heterodimerize with the IGF-I receptor. All receptor combinations bind Insulin, IGF-I, or IGF-II, but with differing affinities. Insulin R signaling regulates glucose uptake and metabolism but also contributes to cell growth, differentiation and apoptosis. Mutations in INSR have been linked to severe insulin resistance (type A and Rabson-Mendenhall syndrome), type II diabetes mellitus, and leprechaunism (Donohue syndrome).

Item	Value
Long Name	Insulin Receptor
Entrez Gene IDs	3643 (Human); 16337 (Mouse)
Alternate Names	CD 220, CD220, CD220 antigen, EC 2.7.10, EC 2.7.10.1, HHF5, INSR, IR, Insulin R, InsulinR, insulin receptor

Citations for Human Total Insulin R DuoSet IC ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total Insulin R DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

Varewijck A et al. (2012-09-07), Circulating insulin-like growth factors may contribute substantially to insulin receptor isoform A and insulin receptor isoform B signalling. Mol Cell Endocrinol, 2012-0365(1):17-24.
PMID: 22982059
Species: Human
Sample Types: Plasma

Holm L et al. (2010-12-20), Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders. PLoS ONE, 2010-15(12):e15606.
PMID: 21187972

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Product Documents for Human Total Insulin R DuoSet IC ELISA

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