Human Tie-2 DuoSet ELISA

Name: Human Tie-2 DuoSet ELISA
Brand: R&D Systems
SKU: DY5159
Price: 745 USD

Catalog # DY5159 | R&D Systems, Inc. a Bio-Techne Brand

Citations (10)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Tie-2 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human Tie-2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Tie-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human Tie-2 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Tie-2 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human Tie-2 DuoSet ELISA

	Human Tie-2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Tie-2

Tie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult endothelial cells, hematopoietic stem cells, and a circulating population of proangiogenic monocytes. Tie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular integrity by mediating endothelial cell-smooth muscle cell communication and inhibiting endothelial cell apoptosis. It is also required for embryonic development of the endocardium. In addition, Tie-2 signaling mediates the quiescence of bone marrow stem cells in response to osteoblast-produced Angiopoietin-1. This quiescence is critical for maintaining an ongoing hematopoietic capability in the bone marrow.

Item	Value
Long Name	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs	7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names	CD202b, CD202b antigen, EC 2.7.10, EC 2.7.10.1, TEK, TEK tyrosine kinase, endothelial, TIE2CD202b, Tie-2, Tie2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, VMCM, VMCM1, angiopoietin-1 receptor, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multiple cutaneous and mucosal

Citations for Human Tie-2 DuoSet ELISA (10)

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Citations are publications that use Bio-Techne products. Selected citations for Human Tie-2 DuoSet ELISA include:

Species: Human
Sample Types: Cell Lysates

P Ku? et al. (2017-02-21), Profiling of selected angiogenesis-related genes in serous ovarian cancer patients Adv Med Sci, 2017-062(1):116-120.
PMID: 28235714
Species: Human
Sample Types: Plasma

Erdman L et al. (2015-09-14), Biomarkers of Host Response Predict Primary End-Point Radiological Pneumonia in Tanzanian Children with Clinical Pneumonia: A Prospective Cohort Study. PLoS ONE, 2015-010(9):e0137592.
PMID: 26366571
Species: Human
Sample Types: Plasma

Ataide R et al. (2015-06-19), Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta. PLoS Negl Trop Dis, 2015-09(6):e0003824.
PMID: 26090803
Species: Human
Sample Types: Serum

Conroy A et al. (2014-01-20), Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-014(0):35.
PMID: 24444080
Species: Human
Sample Types: Serum

Giri H et al. (2013-12-20), Increased endothelial inflammation, sTie-2 and arginase activity in umbilical cords obtained from gestational diabetic mothers. PLoS ONE, 2013-18(12):e84546.
PMID: 24376824
Species: Human
Sample Types: Plasma

Conroy AL et al. (2011-12-12), Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. PLoS ONE, 2011-16(12):e28540.
PMID: 22174834
Species: Human
Sample Types: Plasma

Erdman LK et al. (2011-02-25), Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study. PLoS ONE, 2011-06(2):e17440.
PMID: 21364762
Species: Human
Sample Types: Plasma

Conroy AL et al. (2010-12-29), Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study. PLoS ONE, 2010-15(12):e15291.
PMID: 21209923
Species: Human
Sample Types: Cell Culture Supernates

Lash GE et al. (2010-03-24), Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy. Placenta, 2010-031(6):545-8.
PMID: 20338637
Species: Human
Sample Types: Serum

Szarvas T et al. (2008-09-20), Serum levels of angiogenic factors and their prognostic relevance in bladder cancer. Pathol. Oncol. Res., 2008-015(2):193-201.
PMID: 18807212

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