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Human Phospho-ErbB3/Her3 DuoSet IC ELISA
Catalog # DYC1769-2 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Type: | Solid Phase Sandwich ELISA |
| Sample Types: | Cell Lysates |
| Human Phospho-ErbB3/Her3 DuoSet IC ELISA Features: |
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Figure 1. The Human Phospho-ErbB3 DuoSet IC ELISA is more sensitive than immunoprecipitation (IP)-Western blot analysis The human breast cancer cell line, MDA-MB-453, was treated with 100 ng/mL recombinant HRG-beta 1 (Catalog # 396-HB) for five minutes to induce tyrosine phosphorylation of ErbB3. Serial dilutions of lysates were analyzed by R&D Systems’ Phospho-ErbB3 DuoSet IC ELISA (Catalog # DYC1769) and by IP-Western blot (inset). For IP-Westerns, IPs were done using anti-ErbB3 monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with biotinylated anti-phosphotyrosine monoclonal antibody (Cat # BAM1676) to detect phospho-ErbB3. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by ECL detection. ErbB3 was observed at its predicted size (inset; 185 kDa, arrow) in Western blot analysis. |
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Figure 2. The Human Phospho-ErbB3 DuoSet IC ELISA detects ligand-induced ErbB3 tyrosine phosphorylation MDA-MB-453 cells were untreated or treated with 100 ng/mL of recombinant HRG-beta 1 (Catalog # 396-HB) for five minutes. Cell lysates (100 μg) were analyzed by R&D Systems’ Phospho-ErbB3 DuoSet IC ELISA (Catalog # DYC1769) and by IP-Western blot (inset). IP-Western blots for phospho-ErbB3 (p-ErbB3) were done as described in Figure 1. Blots were stripped and total ErbB3 (ErbB3) was detected using biotinylated anti-ErbB3 polyclonal antibody (Catalog # BAF234). |
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Figure 3. The specificity of the Human Phospho-ErbB3 DuoSet IC ELISA is confirmed by receptor competition MDA-MB-453 cells were treated with 100 ng/mL of recombinant HRG-beta 1 (Catalog # 396-HB) for five minutes. The indicated amounts of ErbB3 (Catalog # 348-ER), ErbB2 (Catalog # 1129-ER) or EGF R (Catalog # 1095-ER) recombinant extracellular domains were added to 5 μg lysate and analyzed using R&D Systems’ Human Phospho-ErbB3 DuoSet IC ELISA (Catalog # DYC1769). Competition was observed only with recombinant ErbB3. |
Citations for Human Phospho-ErbB3/Her3 DuoSet IC ELISA (4)
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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-ErbB3/Her3 DuoSet IC ELISA include:
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Species: Human
Sample Types: Cell Lysates
CD Leitao et al. (2020-06-13), Evaluating the Therapeutic Efficacy of Mono- and Bivalent Affibody-Based Fusion Proteins Targeting HER3 in a Pancreatic Cancer Xenograft Model Pharmaceutics, 2020-012(6).
PMID: 32545760 -
Species: Human
Sample Types: Cell Lysates
Ekerljung L et al. (2012-11-14), The HER2-binding affibody molecule (Z(HER2ratio342))(2) increases radiosensitivity in SKBR-3 cells. PLoS ONE, 2012-17(11):e49579.
PMID: 23166716 -
Species: Human
Sample Types: Cell Lysates
Gostring L et al. (2012-06-29), Cellular Effects of HER3-Specific Affibody Molecules. PLoS ONE, 2012-07(6):e40023.
PMID: 22768204 -
Species: Human
Sample Types: Cell Lysates
Hickinson DM et al. (2010-02-09), AZD8931, an equipotent, reversible inhibitor of signaling by epidermal growth factor receptor, ERBB2 (HER2), and ERBB3: a unique agent for simultaneous ERBB receptor blockade in cancer. Clin. Cancer Res., 2010-016(4):1159-69.
PMID: 20145185
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Customer Reviews for Human Phospho-ErbB3/Her3 DuoSet IC ELISA (1)
FAQs for Human Phospho-ErbB3/Her3 DuoSet IC ELISA
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Which phosphorylated sites are recognized by this assay?
This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
