Which phosphorylated sites are recognized by this assay?

Name: Phospho-FGFR2 alpha DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC684-2
Price: 440 USD

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Phospho-FGFR2 alpha DuoSet IC ELISA

Catalog # DYC684-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Sample Types:	Cell Lysates
Phospho-FGFR2 alpha DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15-(96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Phospho-FGFR2 alpha DuoSet IC ELISA

Summary for Phospho-FGFR2 alpha DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human FGF R2 alpha in cell lysates. An immobilized capture antibody specific for FGF R2 alpha binds both phosphorylated and unphosphorylated FGF R2 alpha. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Phospho-FGFR2 alpha DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Phospho-FGFR2 alpha DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Phospho-FGFR2 alpha DuoSet IC ELISA

	The Human Total-FGF R2 alpha DuoSet IC ELISA is more sensitive than immunoprecipitation (IP)-Western blot analysis Lysates prepared from the human stomach cancer cell line (KatoIII) were diluted in series and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were performed using an anti-FGF R2 alpha monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-FGF R2 alpha monoclonal antibody. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection using WesternGlo Chemiluminescent Detection Substrate (Catalog # AR004). Human FGF R2 alpha can be detected by the Human Total-FGF R2 alpha DuoSet IC ELISA by using approximately 20 to 40 times less lysate than is needed for a conventional IP-Western blot.

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGFR2 alpha

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Item	Value
Long Name	Fibroblast Growth Factor Receptor 2 alpha
Entrez Gene IDs	2263 (Human); 14183 (Mouse)
Alternate Names	BBDS;BEK;BFR-1;CD332;CEK3;CFD1;ECT1;Fibroblast growth factor receptor;Fibroblast growth factor receptor 2;JWS;KGFR;K-SAM;Receptor protein-tyrosine kinase;TK14;TK25, FGF R2a, FGFR2 alpha

Citations for Phospho-FGFR2 alpha DuoSet IC ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Phospho-FGFR2 alpha DuoSet IC ELISA include:

Species: Mouse
Sample Types: Cell Culture Media

PCC Liu et al. (2020-04-21), INCB054828 (pemigatinib), a potent and selective inhibitor of fibroblast growth factor receptors 1, 2, and 3, displays activity against genetically defined tumor models PLoS ONE, 2020-015(4):e0231877.
PMID: 32315352
Species: Human
Sample Types: Cell Culture Supernates

M Su et al. (2017-11-23), Andrographolide enhanced 5-fluorouracil-induced antitumor effect in colorectal cancer via inhibition of c-MET pathway Drug Des Devel Ther, 2017-111(0):3333-3341.
PMID: 29200829

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FAQs for Phospho-FGFR2 alpha DuoSet IC ELISA

Which phosphorylated sites are recognized by this assay?

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Phospho-FGFR2 alpha DuoSet IC ELISA

Catalog # DYC684-2 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Summary for Phospho-FGFR2 alpha DuoSet IC ELISA

Product Specifications for Phospho-FGFR2 alpha DuoSet IC ELISA

Kit Contents for Phospho-FGFR2 alpha DuoSet IC ELISA

Other Reagents Required

Scientific Data Examples for Phospho-FGFR2 alpha DuoSet IC ELISA

Preparation and Storage

Background: FGFR2 alpha

Citations for Phospho-FGFR2 alpha DuoSet IC ELISA (2)

FAQs for Phospho-FGFR2 alpha DuoSet IC ELISA

Product Documents for Phospho-FGFR2 alpha DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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