Human EGFR Quantikine ELISA Kit Best Seller

Name: Human EGFR Quantikine ELISA Kit
Brand: R&D Systems
SKU: DEGFR0
Rating: 3 (1 reviews)

Catalog # DEGFR0 | R&D Systems, Inc. a Bio-Techne Brand

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Citations (4)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Human Milk (50 uL)
Sensitivity:	0.036 ng/mL
Assay Range:	0.313 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)
Human EGFR Quantikine ELISA Kit Summary:	The Quantikine Human EGF R immunoassay is a 4.5 hour solid phase ELISA designed to measure EGF R in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human EGF R and has been shown to accurately quantitate the recombinant factor. Results obtained using natural EGF R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human EGF R.

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Product Specifications for Human EGFR Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human EGF R
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	1.65	5.08	10.6	1.63	5	10
Standard Deviation	0.094	0.17	0.507	0.144	0.416	0.816
CV%	5.7	3.3	4.8	8.8	8.3	8.2

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	1.69	5.33	11.2	1.7	5.21	10.4
Standard Deviation	0.072	0.199	0.616	0.161	0.522	1.03
CV%	4.3	3.7	5.5	9.4	10	10

Recovery

The recovery of EGF R spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	86-106
EDTA Plasma (n=4)	99	91-108
Heparin Plasma (n=4)	101	92-111
Serum (n=4)	101	89-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of EGF R were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human EGFR Quantikine ELISA Kit

	Human EGF R/ErbB1 ELISA Cell Culture Supernate/Human Milk Standard Curve
	Human EGF R/ErbB1 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGFR

The EGF R subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular ligand binding domain containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites. The human EGF R cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain. Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse. Within the ECD, human EGF R shares 88% aa sequence identity with mouse and rat EGF R. It shares 43% - 44% aa sequence identity with the ECD of human ErbB2, ErbB3, and ErbB4. EGF R binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGF R is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs.

Item	Value
Long Name	Epidermal Growth Factor Receptor
Entrez Gene IDs	1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EGF R, EGFR, ERBB1PIG61, ErbB, ErbB1, HER-1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), mENA

Citations for Human EGFR Quantikine ELISA Kit (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human EGFR Quantikine ELISA Kit include:

Species: Human
Sample Types: Plasma

M Kyohara et al. (2020-09-29), Soluble EGFR, a hepatokine, and adipsin, an adipokine, are biomarkers correlated with distinct aspects of insulin resistance in type 2 diabetes subjects Diabetol Metab Syndr, 2020-012(0):83.
PMID: 33005239
Species: Human
Sample Types: Serum

Aderhold C et al. (2014-07-04), Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study. Oncol Lett, 2014-08(3):1112-1118.
PMID: 25120668
Species: Human
Sample Types: Cell Culture Supernates

Garnier et al. (2012-11-01), Cancer cells induced to express mesenchymal phenotype release exosome-like extracellular vesicles carrying tissue factor. J Biol Chem, 2012-1287(52):43565-72.
PMID: 23118232
Species: Human
Sample Types: Plasma

Korostishevsky M et al. (2010-03-16), Morphological and biochemical features of obesity are associated with mineralization genes' polymorphisms. Int J Obes (Lond), 2010-034(8):1308-18.
PMID: 20231843

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Human EGFR Quantikine ELISA Kit

Name: Rahul Mallick

Sample Tested: HUVEC human umbilical vein endothelial cells

Verified Customer | Posted 08/26/2020

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Product Documents for Human EGFR Quantikine ELISA Kit

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COA

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.