Human IGF-I R/IGF1R DuoSet ELISA

Name: Human IGF-I R/IGF1R DuoSet ELISA
Brand: R&D Systems
SKU: DY391
Price: 745 USD

Catalog # DY391 | R&D Systems, Inc. a Bio-Techne Brand

Citations (6)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	250 - 16,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human IGF-I R/IGF1R DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Human IGF-I R/IGF1R DuoSet ELISA

Summary for Human IGF-I R/IGF1R DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IGF-I R. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human IGF-I R/IGF1R DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human IGF-I R/IGF1R DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human IGF-I R/IGF1R DuoSet ELISA

	Human IGF-I R ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues.

IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment segment and a 124 aa cytoplasmic tail. IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, It would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes.

The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligandbinding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF-I R stimulates intrinsic kinase activity.

Item	Value
Long Name	Insulin-like Growth Factor I Receptor
Entrez Gene IDs	3480 (Human)
Alternate Names	CD221, CD221 antigen, EC 2.7.10, EC 2.7.10.1, IGF-1R, IGF-I R, IGF-I receptor, IGF-IR, IGF1R, IGFIR, IGFR, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216, insulin-like growth factor 1 receptor, soluble IGF1R variant 1, soluble IGF1R variant 2

Citations for Human IGF-I R/IGF1R DuoSet ELISA (6)

Showing 1 - 6 of 6 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human IGF-I R/IGF1R DuoSet ELISA include:

Species: Human
Sample Types: Serum

A Harangu? et al. (2022-12-13), Identification of Potential microRNA Panels for Male Non-Small Cell Lung Cancer Identification Using Microarray Datasets and Bioinformatics Methods Journal of personalized medicine, 2022-112(12).
PMID: 36556276
Species: Complex Species Category
Sample Types: Plasma

G Vaniotis et al. (2018-11-26), Enhanced anti-metastatic bioactivity of an IGF-TRAP re-engineered to improve physicochemical properties Sci Rep, 2018-18(1):17361.
PMID: 30478273
Species: Human
Sample Types: Cell Culture Supernates

Fluhr H et al. (2009-12-03), Heparin and low-molecular-weight heparins modulate the decidualization of human endometrial stromal cells. Fertil. Steril., 2009-193(8):2581-7.
PMID: 19962139
Species: Human
Sample Types: Plasma

Pitteri SJ et al. (2009-11-19), Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery. PLoS ONE, 2009-14(11):e7916.
PMID: 19936259
Species: Human
Sample Types: Plasma

Wang N et al. (2009-04-14), Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases. Mol. Ther., 2009-017(7):1241-9.
PMID: 19367255
Species: Human
Sample Types: Cell Culture Supernates

Shi S et al. (2009-01-09), Growth factor regulation of growth factors in articular chondrocytes. J. Biol. Chem., 2009-0284(11):6697-704.
PMID: 19136669

There are no citations that match your criteria.

Showing 1 - 6 of 6 citations Showing All

Product Documents for Human IGF-I R/IGF1R DuoSet ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS