Human Total FGFR4 DuoSet IC ELISA

Standard Curve

The Human Total FGF R4 DuoSet® IC ELISA is more sensitive than immunoprecipitation (IP)-Western blot analysis. Lysates prepared from MDA-MB-453 human breast cancer cells were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-FGF R4 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-FGF R4 monoclonal antibody to detect total FGF R4. Bands were visualized by chemiluminescent detection. Human FGF R4 can be detected by this DuoSet IC ELISA by using approximately 2 to 4 times less lysate than is needed for a conventional IP-Western blot.

The Human Total FGF R4 DuoSet® IC ELISA measures the relative level of FGF R4. Lysates were prepared from MDA-MB-453 human breast cancer cells and HEK293 human embryonic kidney cells transfected with human FGF R4 (HEK293-hFGF R4). ELISA was performed using 100 μg of the MDA-MB-453 cell lysate and 5 μg of the HEK293-hFGF R4 cell lysate. IP-Western blot analyses were performed as described in Figure 1, using 400 μg of cell lysate.