Human Total DDR1 DuoSet IC ELISA

Name: Human Total DDR1 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC2396-2
Rating: 4 (1 reviews)

Catalog # DYC2396-2 | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	312.5 - 20,000 pg/mL
Sample Types:	Cell Lysates
Human Total DDR1 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total DDR1 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total DDR1 in cell lysates. An immobilized capture antibody specific for DDR1 binds both phosphorylated and unphosphorylated DDR1. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total DDR1 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total DDR1 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total DDR1 DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DDR1

DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120-140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases and serve as receptors for collagen. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers. Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region. Two additional kinase deficient splice forms are expressed in colon cancer. The discoidin-like domain mediates binding to collagens I-V. DDR1 selectively recognizes the triple helical structure of collagen. It is expressed on the cell surface as a dimer which can include different isoforms. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization. Collagen binding induces prolonged autophosphorylation, including the NPxY motif. Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment. TIMP-3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF. Overexpression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not.

Item	Value
Long Name	Discoidin Domain Receptor 1
Entrez Gene IDs	780 (Human)
Alternate Names	CAK, CD167 antigen-like family member A, CD167a, CD167a antigen, DDR, DDR1, Discoidin receptor tyrosine kinase, EC 2.7.10, EC 2.7.10.1, EDDR1, ENTRK4, Epithelial discoidin domain receptor 1, HGK2, MCK-10, MCK10, NTRK4, PTK3A, PTK3A protein tyrosine kinase 3A, Protein-tyrosine kinase 3A, RTK6, TRK E, TrkE, discoidin domain receptor family, member 1, discoidin domain receptor tyrosine kinase 1, receptor, type 4

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Human Total DDR1 DuoSet IC ELISA

Name: Anonymous

Sample Tested: Human cells,Human tissue

Verified Customer | Posted 08/08/2019

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Product Documents for Human Total DDR1 DuoSet IC ELISA

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Human Total DDR1 DuoSet IC ELISA

Catalog # DYC2396-2 | R&D Systems, Inc. a Bio-Techne Brand

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Summary for Human Total DDR1 DuoSet IC ELISA

Product Specifications for Human Total DDR1 DuoSet IC ELISA

Kit Contents for Human Total DDR1 DuoSet IC ELISA

Other Reagents Required

Scientific Data Examples for Human Total DDR1 DuoSet IC ELISA

Preparation and Storage

Background: DDR1

Citations for Human Total DDR1 DuoSet IC ELISA

Customer Reviews for Human Total DDR1 DuoSet IC ELISA (1)

Customer Images

Human Total DDR1 DuoSet IC ELISA

Product Documents for Human Total DDR1 DuoSet IC ELISA

Product Datasheets

COA

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SDS

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