Which phosphorylated sites are recognized by this assay?

Name: Human Phospho-Insulin R DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC2718-2
Price: 440 USD

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-Insulin R DuoSet IC ELISA

Catalog # DYC2718-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Sample Types:	Cell Lysates
Human Phospho-Insulin R DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15-(96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Phospho-Insulin R DuoSet IC ELISA

Summary for Human Phospho-Insulin R DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human Insulin R / CD220 in cell lysates. An immobilized capture antibody specific for Insulin R / CD220 binds both phosphorylated and unphosphorylated Insulin R / CD220. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Human Phospho-Insulin R DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Phospho-Insulin R DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Insulin R/CD220

Insulin Receptor (INSR, CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases. The mature receptor contains an extracellular alpha subunit and an extracellular/transmembrane/cytoplasmic beta subunit. Alternative splicing generates A and B isoforms which may homodimerize or heterodimerize with the IGF-I receptor. All receptor combinations bind Insulin, IGF-I, or IGF-II, but with differing affinities. Insulin R signaling regulates glucose uptake and metabolism but also contributes to cell growth, differentiation and apoptosis. Mutations in INSR have been linked to severe insulin resistance (type A and Rabson-Mendenhall syndrome), type II diabetes mellitus, and leprechaunism (Donohue syndrome).

Item	Value
Long Name	Insulin Receptor
Entrez Gene IDs	3643 (Human); 16337 (Mouse)
Alternate Names	CD 220, CD220, CD220 antigen, EC 2.7.10, EC 2.7.10.1, HHF5, INSR, IR, Insulin R, InsulinR, insulin receptor

Citations for Human Phospho-Insulin R DuoSet IC ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-Insulin R DuoSet IC ELISA include:

Species: Human
Sample Types: Tissue Homogenates

Lo et al. (2014-08-18), A novel glycated hemoglobin A1c-lowering traditional Chinese medicinal formula, identified by translational medicine study. PLoS ONE, 2014-09(8):e104650.
PMID: 25133699
Species: Human
Sample Types: Cell Lysates

Brouwer-Visser et al. (2014-06-16), Insulin-like growth factor 2 silencing restores taxol sensitivity in drug resistant ovarian cancer. PLoS ONE, 2014-09(6):e100165.
PMID: 24932685
Species: Human
Sample Types: Cell Lysates

Weichhaus M et al. (2012-05-16), A novel role for insulin resistance in the connection between obesity and postmenopausal breast cancer. Int. J. Oncol., 2012-041(2):745-52.
PMID: 22614942

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FAQs for Human Phospho-Insulin R DuoSet IC ELISA

Which phosphorylated sites are recognized by this assay?

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-Insulin R DuoSet IC ELISA

Catalog # DYC2718-2 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Summary for Human Phospho-Insulin R DuoSet IC ELISA

Product Specifications for Human Phospho-Insulin R DuoSet IC ELISA

Kit Contents for Human Phospho-Insulin R DuoSet IC ELISA

Other Reagents Required

Preparation and Storage

Background: Insulin R/CD220

Citations for Human Phospho-Insulin R DuoSet IC ELISA (3)

FAQs for Human Phospho-Insulin R DuoSet IC ELISA

Product Documents for Human Phospho-Insulin R DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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