Which phosphorylated sites are recognized by this assay?

Name: Human Phospho-EphB4 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC4057-2
Price: 440 USD

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-EphB4 DuoSet IC ELISA

Catalog # DYC4057-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Sample Types:	Cell Lysates
Human Phospho-EphB4 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15-(96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Phospho-EphB4 DuoSet IC ELISA

Summary for Human Phospho-EphB4 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human EphB4 in cell lysates. An immobilized capture antibody specific for EphB4 binds both phosphorylated and unphosphorylated EphB4. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Human Phospho-EphB4 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Phospho-EphB4 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EphB4

Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-B ligands are referred to as EphB. All Eph receptors contain an N-terminal Ig-like domain, a cysteine-rich region with 19 conserved cysteines and two fibronectin type III domains. The cytoplasmic region contains a typical tyrosine kinase organization.

Item	Value
Long Name	Eph Receptor B4
Entrez Gene IDs	2050 (Human); 13846 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, HTKephrin receptor EphB4, Htk, Mdk2, Myk1, Tyro11, Tyrosine-protein kinase TYRO11, Tyrosine-protein kinase receptor HTK, ephrin type-B receptor 4, hepatoma transmembrane kinase, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3

Citations for Human Phospho-EphB4 DuoSet IC ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-EphB4 DuoSet IC ELISA include:

Species: Human

C You et al. (2017-04-01), EphB4 forward signalling mediates angiogenesis caused by CCM3/PDCD10-ablation J. Cell. Mol. Med., 2017-00(0).
PMID: 28371279
Species: Human
Sample Types: Tissue Homogenates

(2016-12-12), Enhancing radiosensitization in EphB4 receptor-expressing Head and Neck Squamous Cell Carcinomas Sci Rep, 2016-16(0):38792.
PMID: 27941840
Species: Human
Sample Types: Cell Lysates

Giorgio C et al. (2011-03-30), Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation. PLoS ONE, 2011-06(3):e18128.
PMID: 21479221
Species: Human
Sample Types: Cell Lysates

Pennisi A et al. (2009-07-13), The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth. Blood, 2009-0114(9):1803-12.
PMID: 19597185

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FAQs for Human Phospho-EphB4 DuoSet IC ELISA

Which phosphorylated sites are recognized by this assay?

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-EphB4 DuoSet IC ELISA

Catalog # DYC4057-2 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Summary for Human Phospho-EphB4 DuoSet IC ELISA

Product Specifications for Human Phospho-EphB4 DuoSet IC ELISA

Kit Contents for Human Phospho-EphB4 DuoSet IC ELISA

Other Reagents Required

Preparation and Storage

Background: EphB4

Citations for Human Phospho-EphB4 DuoSet IC ELISA (4)

FAQs for Human Phospho-EphB4 DuoSet IC ELISA

Product Documents for Human Phospho-EphB4 DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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