Human Total M-CSF R/CD115 DuoSet IC ELISA

Name: Human Total M-CSF R/CD115 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC329-2

Catalog # DYC329-2 | R&D Systems, Inc. a Bio-Techne Brand

Product Datasheet / COA / SDS

Discontinued Product

DYC329-2 has been discontinued. View all M-CSF R/CD115 products.

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	312 - 20,000 pg/mL
Sample Types:	Cell Lysates
Human Total M-CSF R/CD115 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Total M-CSF R/CD115 DuoSet IC ELISA

Technical Data
Citations / Reviews

Summary for Human Total M-CSF R/CD115 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total M-CSF R/CD115 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total M-CSF R/CD115 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF R/CD115

M-CSF R/CD115, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes c-kit, the receptor for SCF, and the alpha and beta receptors for PDGF. M-CSF R/CD115 is expressed primarily on cells of the monocyte/macrophage lineage and in various tissues of the developing placenta.

Item	Value
Long Name	Macrophage Colony Stimulating Factor Receptor
Entrez Gene IDs	1436 (Human); 12978 (Mouse)
Alternate Names	CD115, CD115 antigen, CSF-1-R, CSF1R, CSFR, EC 2.7.10.1, FMS proto-oncogene, FMSFIM2, M-CSF R, M-CSFR, MCSFR, McDonough feline sarcoma viral (v-fms) oncogene homolog, Proto-oncogene c-Fms, c-fms, colony stimulating factor 1 receptor, macrophage colony stimulating factor I receptor, macrophage colony-stimulating factor 1 receptor

Citations for Human Total M-CSF R/CD115 DuoSet IC ELISA

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Human Total M-CSF R/CD115 DuoSet IC ELISA

Catalog # DYC329-2 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Human Total M-CSF R/CD115 DuoSet IC ELISA

Product Specifications for Human Total M-CSF R/CD115 DuoSet IC ELISA

Kit Contents for Human Total M-CSF R/CD115 DuoSet IC ELISA

Other Reagents Required

Preparation and Storage

Background: M-CSF R/CD115

Citations for Human Total M-CSF R/CD115 DuoSet IC ELISA

Product Documents for Human Total M-CSF R/CD115 DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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