Human Total PDGF R alpha DuoSet IC ELISA

Name: Human Total PDGF R alpha DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC322-2
Price: 440 USD

Catalog # DYC322-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	312 - 20,000 pg/mL
Sample Types:	Cell Lysates
Human Total PDGF R alpha DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total PDGF R alpha DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total PDGF R alpha in cell lysates. An immobilized capture antibody specific for PDGF R alpha binds both phosphorylated and unphosphorylated PDGF R alpha. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total PDGF R alpha DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total PDGF R alpha DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total PDGF R alpha DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF R alpha

PDGF R alpha (Platelet-Derived Growth Factor Receptor alpha) is a transmembrane receptor tyrosine kinase that can form homodimers or heterodimers with PDGF R beta when engaged by dimers of the PDGF ligands. PDGF R alpha binds to PDGF-AA, PDGF-AB, and PDGF-CC. It is strongly expressed in oligodendrocyte, lung, skin, and intestinal progenitor cells, at low levels in most mesenchymal cells, and can be induced by inflammation. PDGF R alpha function is important during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme. Dysregulated PDGF R alpha signaling can result in spina bifida or cleft palate-type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart, and skin (scleroderma).

Item	Value
Long Name	Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs	5156 (Human); 18595 (Mouse)
Alternate Names	CD140 antigen-like family member A, CD140a, CD140a antigen, EC 2.7.10, EC 2.7.10.1, MGC74795, PDGF R alpha, PDGF-R-alpha, PDGFR alpha, PDGFR2, PDGFRA, PDGFRA/BCR fusion, RHEPDGFRA, alpha-type platelet-derived growth factor receptor, platelet-derived growth factor receptor, alpha polypeptide, rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein

Citations for Human Total PDGF R alpha DuoSet IC ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total PDGF R alpha DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

Aderhold C et al. (2013-12-01), Small molecule-based chemotherapeutic approach in p16-positive and -negative HNSCC in vitro. Anticancer Res, 2013-133(12):5385-93.
PMID: 24324073

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Product Documents for Human Total PDGF R alpha DuoSet IC ELISA

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