Human Total TrkB DuoSet IC ELISA

Name: Human Total TrkB DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC397-2
Price: 440 USD

Catalog # DYC397-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	93.8 - 6,000 pg/mL
Sample Types:	Cell Lysates
Human Total TrkB DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total TrkB DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total TrkB in cell lysates. An immobilized capture antibody specific for TrkB binds both phosphorylated and unphosphorylated TrkB. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total TrkB DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total TrkB DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total TrkB DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TrkB

Members of the Trk family (TrkA, B, and C) of receptor tyrosine kinases act as receptors for the neurotrophin family: BDNF, NGF, NT-3, and NT-4. Trks exhibit selectivity for specific neurotrophins. TrkA, TrkB and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a common structural organization consisting of an extracellular region with two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains, and an intracellular region that contains the tyrosine kinase domain. They are best known for their regulation of neuronal survival and axon outgrowth.

Item	Value
Long Name	Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs	4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names	BDNF/NT-3 growth factors receptor, EC 2.7.10, EC 2.7.10.1, GP145-TrkB, NTRK2, Neurotrophic tyrosine kinase receptor type 2, TRKBBDNF-tropomyosine receptor kinase B, TrkB, TrkB tyrosine kinase, neurotrophic tyrosine kinase, receptor, type 2, trk-B, tyrosine kinase receptor B

Citations for Human Total TrkB DuoSet IC ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total TrkB DuoSet IC ELISA include:

Species: Human
Sample Types: Plasma

C Wu et al. (2020-05-12), Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia J Psychiatr Res, 2020-0126(0):92-97.
PMID: 32428748
Species: Human
Sample Types: Serum

H Jiang et al. (2017-04-04), The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment Transl Psychiatry, 2017-07(4):e1079.
PMID: 28375203

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Product Documents for Human Total TrkB DuoSet IC ELISA

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