Human Tie-2 Quantikine ELISA Kit

Name: Human Tie-2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DTE200

Catalog # DTE200 | R&D Systems, Inc. a Bio-Techne Brand

Citations (25)

Product Datasheet / COA / SDS

New Version Available - DY5159

Discontinued Product

DTE200 has been discontinued and is replaced by DY5159.

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.066 ng/mL
Assay Range:	0.156 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human Tie-2 Quantikine ELISA Kit Summary:	The Quantikine Human Tie-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Tie-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Tie-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Tie-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Tie-2.

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Technical Data
Citations (25) / Reviews

Product Specifications for Human Tie-2 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human Tie-2
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (ng/mL)	0.76	2.57	5.14	0.82	2.65	5.15
Standard Deviation	0.04	0.11	0.26	0.07	0.17	0.27
CV%	5.3	4.3	5.1	8.5	6.4	5.2

Recovery

The recovery of Tie-2 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	91	85-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Tie-2 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Tie-2 Quantikine ELISA Kit

	Human Tie-2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Tie-2

Tie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult endothelial cells, hematopoietic stem cells, and a circulating population of proangiogenic monocytes. Tie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular integrity by mediating endothelial cell-smooth muscle cell communication and inhibiting endothelial cell apoptosis. It is also required for embryonic development of the endocardium. In addition, Tie-2 signaling mediates the quiescence of bone marrow stem cells in response to osteoblast-produced Angiopoietin-1. This quiescence is critical for maintaining an ongoing hematopoietic capability in the bone marrow.

Item	Value
Long Name	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs	7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names	CD202b, CD202b antigen, EC 2.7.10, EC 2.7.10.1, TEK, TEK tyrosine kinase, endothelial, TIE2CD202b, Tie-2, Tie2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, VMCM, VMCM1, angiopoietin-1 receptor, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multiple cutaneous and mucosal

Citations for Human Tie-2 Quantikine ELISA Kit (25)

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Citations are publications that use Bio-Techne products. Selected citations for Human Tie-2 Quantikine ELISA Kit include:

JV Moxon et al. (2022-11-04), Serum angiopoietin-1 concentration does not distinguish patients with ischaemic stroke from those presenting to hospital with ischaemic stroke mimics BMC cardiovascular disorders, 2022-122(1):462.
PMID: 36333663
Species: Human
Sample Types: Serum

J Kaur et al. (2022-06-03), Cholecalciferol supplementation and angiogenic markers in chronic kidney disease PLoS ONE, 2022-017(6):e0268946.
PMID: 35657784
Species: Human
Sample Types: Plasma

NAM Dekker et al. (2019-04-11), Microcirculatory perfusion disturbances following cardiac surgery with cardiopulmonary bypass are associated with in vitro endothelial hyperpermeability and increased angiopoietin-2 levels Crit Care, 2019-023(1):117.
PMID: 30975180
Species: Human
Sample Types: Serum

Y Shi et al. (2018-05-23), Different patterns of inflammatory and angiogenic factors are associated with peritoneal small solute transport and peritoneal protein clearance in peritoneal dialysis patients BMC Nephrol, 2018-019(1):119.
PMID: 29792163
Species: Human
Sample Types: Serum

F Moritz et al. (2017-05-25), Tie2 as a novel key factor of microangiopathy in systemic sclerosis Arthritis Res. Ther., 2017-019(1):105.
PMID: 28545512
Species: Human
Sample Types: Plasma

D Gozal et al. (2017-05-05), Angiopoietin-2 and soluble Tie-2 receptor plasma levels in children with obstructive sleep apnea and obesity Obesity (Silver Spring), 2017-00(0).
PMID: 28474375
Species: Human
Sample Types: Cell Lysates

(2016-04-01), TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1 Sci Adv, 2016-02(4):e1501290.
PMID: 27757426
Species: Human
Sample Types: Plasma

Jongman R et al. (2015-08-26), Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass. PLoS ONE, 2015-010(8):e0136205.
PMID: 26309217
Species: Human
Sample Types: Serum

Wertenbroek et al. (2013-05-30), Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases. BMC Cancer, 2013-013(1):266.
PMID: 23721455
Species: Human
Sample Types: Plasma

Rasul S et al. (2011-06-23), Circulating angiopoietin-2 and soluble Tie-2 in type 2 diabetes mellitus: a cross-sectional study. Cardiovasc Diabetol, 2011-010(0):55.
PMID: 21699724
Species: Human
Sample Types: Serum

Koenecke C et al. (2010-05-01), Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML. Ann. Hematol., 2010-089(5):459-67.
PMID: 20054548
Species: Human
Sample Types: Tissue Homogenates

Boucherat O et al. (2010-03-26), Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF. Am. J. Physiol. Lung Cell Mol. Physiol., 2010-0298(6):L849-56.
PMID: 20348277
Species: Human
Sample Types: Plasma

Serefhanoglu S et al. (2008-12-11), Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors. Ann. Hematol., 2008-188(7):667-71.
PMID: 19082594
Species: Bovine
Sample Types: Cell Lysates

Cai J et al. (2008-05-01), The angiopoietin/Tie-2 system regulates pericyte survival and recruitment in diabetic retinopathy. Invest. Ophthalmol. Vis. Sci., 2008-049(5):2163-71.
PMID: 18436850
Species: Human
Sample Types: Plasma

Lee CY et al. (2007-09-11), Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia. Br. J. Cancer, 2007-097(7):877-82.
PMID: 17848952
Species: Human
Sample Types: Serum

Niedzwiecki S et al. (2007-03-19), Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers. Cytokine, 2007-036(5):291-5.
PMID: 17374490
Species: Human
Sample Types: Plasma

Caine GJ et al. (2007-01-19), Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients. Cancer Lett., 2007-0251(2):296-301.
PMID: 17240049
Species: Human
Sample Types: Serum

Quartarone E et al. (2006-09-15), Differential levels of soluble angiopoietin-2 and Tie-2 in patients with haematological malignancies. Eur. J. Haematol., 2006-077(6):480-5.
PMID: 16978237
Molskness TA et al. (2006-09-14), Circulating levels of total angiopoietin-2 and the soluble Tie-2 receptor in women during ovarian stimulation and early gestation. Fertil. Steril., 2006-086(5):1531-3.
PMID: 16978614
Species: Human
Sample Types: Plasma

Caine GJ et al. (2006-08-07), Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer. Cancer Lett., 2006-0248(1):131-6.
PMID: 16891056
Species: Human
Sample Types: Serum

Koutroubakis IE et al. (2006-02-01), Potential role of soluble angiopoietin-2 and Tie-2 in patients with inflammatory bowel disease. Eur. J. Clin. Invest., 2006-036(2):127-32.
PMID: 16436095
Species: Human
Sample Types: Tissue Homogenates

Tseng JJ et al. (2006-02-01), Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta. Am. J. Obstet. Gynecol., 2006-0194(2):564-71.
PMID: 16458662
Species: Human
Sample Types: Cell Lysates

Holland SJ et al. (2005-10-15), Multiple roles for the receptor tyrosine kinase axl in tumor formation. Cancer Res., 2005-165(20):9294-303.
PMID: 16230391
Species: Human
Sample Types: Serum

Mross K et al. (2005-06-01), Phase I clinical and pharmacokinetic study of PTK/ZK, a multiple VEGF receptor inhibitor, in patients with liver metastases from solid tumours. Eur. J. Cancer, 2005-041(9):1291-9.
PMID: 15939265
Species: Human
Sample Types: Cell Culture Supernates

Stahl A et al. (2004-09-17), Bi-directional cell contact-dependent regulation of gene expression between endothelial cells and osteoblasts in a three-dimensional spheroidal coculture model. Biochem. Biophys. Res. Commun., 2004-0322(2):684-92.
PMID: 15325284

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Product Documents for Human Tie-2 Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.