Human Total ErbB4/Her4 DuoSet IC ELISA

Standard Curve

The Human Total ErbB4 DuoSet IC ELISA is more sensitive than Western Blot analysis Lysates prepared from MDA-MB-453 human breast cancer cells were serially diluted and analyzed by (A) Western Blot and (B) this DuoSet IC ELISA. Immunoblots were incubated with an anti-ErbB4 polyclonal antibody to detect total ErbB4. Bands were visualized with anti-rabbit IgG-HRP (Catalog # HAF008) followed by chemiluminescent detection. Human ErbB4 can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western Blot.

The Human Total ErbB4 DuoSet^® IC ELISA measures the relative level of ErbB4. Lysates were prepared from MDA-MB-453 human breast cancer cells and the MCF-7 human breast cancer cell line. Both ELISA and Western Blot (inset) analyses were performed using 100 μg of lysate.