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Rabbit IgG Horseradish Peroxidase-conjugated Antibody Best Seller

Catalog # HAF008 | R&D Systems, Inc. a Bio-Techne Brand
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HAF008

Key Product Details

Species Reactivity

Rabbit

Applications

Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Product Summary for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Immunogen

Rabbit IgG

Specificity

Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Detection of Rabbit IgG Primary Antibody by Western Blot

Detection of Rabbit IgG Primary Antibody by Western Blot

Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of FoxP3 in Human Tonsil

Detection of FoxP3 in human tonsil using 5 μg/mL of Rabbit monoclonal anti-human FoxP3 primary antibody (MAB8214) followed by 30 min incubation with Goat anti Rabbit HRP conjugated secondary antibodies (Catalog # HAF008) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.

Applications for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Use at a 1:20 dilution 

Western Blot

Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 5 using HAF008 in the following applications:

Published Applications

Read 113 publications using HAF008 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG

Product Documents for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

For research use only

Citations for Rabbit IgG Horseradish Peroxidase-conjugated Antibody (113)

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Customer Reviews for Rabbit IgG Horseradish Peroxidase-conjugated Antibody (5)

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  • Rabbit IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line and NIH-3T3 mouse embryonic fibroblast cell line
    Species: Human
    Verified Customer | Posted 04/12/2021
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver cells
    Species: Mouse
    Verified Customer | Posted 10/27/2019
    1:1000 Dilution in 5% nonfat dry milk in TBST for 1 hr at RT.
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Liver tissue, Skin tissue, Muscle tissue and human liver cells, Sample Amount: 15 ug
    Species: Human and Pig
    Verified Customer | Posted 08/14/2019
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG HRP-conjugated Antibody
    Name: Danlee Enzler
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 04/24/2018
  • Rabbit IgG HRP-conjugated Antibody
    Name: Adrie Jones
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Human
    Verified Customer | Posted 10/31/2017

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FAQs for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

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  • Q: May I know the buffer used in this product?

    A: It is supplied as a 0.2um filtered solution in sodium phosphate and Proclin 300

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