Human/Mouse/Rat HSP60 Antibody

Name: Human/Mouse/Rat HSP60 Antibody
Brand: R&D Systems
SKU: AF1800
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1800

Citations (2)

(1)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human HSP60 Met1-Phe573 Accession # P10809
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Rabbit IgG
Specificity:	Detects human, mouse, and rat HSP60 in Western blots.

View Full Technical Details for Human/Mouse/Rat HSP60 Antibody

Applications for Human/Mouse/Rat HSP60 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Simple Western	0.5 - 1 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat HSP60 Antibody

	Western Blot Detection of Human/Mouse/Rat HSP60 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP60 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human and Mouse HSP60 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 at approximately 60 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP60 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Simple Western Detection of Human, Mouse, and Rat HSP60 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 at approximately 59 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP60 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen and protein A Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: HSP60

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat-shock or other cellular stress. Full length human HSP60 is 98% identical to mouse and rat HSP60.

Item	Value
Long Name	Heat Shock Protein 60
Entrez Gene IDs	3329 (Human); 110907 (Mouse); 63868 (Rat)
Alternate Names	60 kDa chaperonin, Chaperonin 60, GROEL, HLD4, HSP-60, HSP60, HSP60SPG13, HSP65, HSPD1, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, SPG13, cpn60, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), heat shock protein 65, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)

Citations for Human/Mouse/Rat HSP60 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat HSP60 Antibody include:

Species: Human
Sample Types: Tissue Lysates
Applications: Simple Western

JM Collins et al. (2021), Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary western blot technology and total protein normalization Journal of pharmacological and toxicological methods, 112(0):107117.
PMID: 34474151
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Abdrabou et al. (2020), Differential Subcellular Distribution and Translocation of Seven 14-3-3 Isoforms in Response to EGF and During the Cell Cycle Int J Mol Sci, 21(1).
PMID: 31906564

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Customer Reviews for Human/Mouse/Rat HSP60 Antibody (1)

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Human/Mouse/Rat HSP60 Antibody

Name: Khuynh Nguyen

Application: WB

Sample Tested: MDA-MB-231 human breast cancer cell line

Species: Human

Verified Customer | Posted 04/18/2022

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Product Documents for Human/Mouse/Rat HSP60 Antibody

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Human/Mouse/Rat HSP60 Antibody

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Applications for Human/Mouse/Rat HSP60 Antibody

Scientific Data Examples for Human/Mouse/Rat HSP60 Antibody

Western Blot

Simple Western

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: HSP60

Citations for Human/Mouse/Rat HSP60 Antibody (2)

Customer Reviews for Human/Mouse/Rat HSP60 Antibody (1)

Customer Images

Human/Mouse/Rat HSP60 Antibody

Product Documents for Human/Mouse/Rat HSP60 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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