Human CD117/c-kit Quantikine ELISA Kit

Name: Human CD117/c-kit Quantikine ELISA Kit
Brand: R&D Systems
SKU: DSCR00

Catalog # DSCR00 | R&D Systems, Inc. a Bio-Techne Brand

Citations (6)

Product Datasheet / COA / SDS

Discontinued Product

DSCR00 has been discontinued. View all CD117/c-kit products.

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity:	0.339 ng/mL
Assay Range:	1.56 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human CD117/c-kit Quantikine ELISA Kit Summary:	The Quantikine Human SCF sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SCF sR in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human SCF sR and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SCF sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SCF sR.

View Full Technical Details for Human CD117/c-kit Quantikine ELISA Kit

Technical Data
Citations (6) / Reviews

Product Specifications for Human CD117/c-kit Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human SCF sR
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (ng/mL)	5.68	13.8	26.4	5.32	13.6	26.9
Standard Deviation	0.228	0.39	0.707	0.481	0.509	1.21
CV%	4	2.8	2.7	9	3.7	4.5

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (ng/mL)	4.69	14.1	25.7	5.9	15.1	28.7
Standard Deviation	0.298	0.359	0.562	0.555	0.688	1.76
CV%	6.4	2.5	2.2	9.4	4.6	6.1

Recovery

The recovery of SCF sR spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	97-110
EDTA Plasma (n=4)	91	84-106
Heparin Plasma (n=4)	92	87-102
Serum (n=4)	93	86-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SCF sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human CD117/c-kit Quantikine ELISA Kit

	Human CD117/c-kit ELISA Serum/Plasma Standard Curve
	Human CD117/c-kit ELISA Cell Culture Supernate Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD117/c-kit

CD117, also known as c-kit and SCF R, is the cellular receptor for Stem Cell Factor (SCF), also named c-kit ligand, mast cell growth factor, and steel factor. This receptor-ligand system plays essential roles in germ cell development, melanogenesis, hematopoiesis, and oncogenesis. CD117 is expressed on lymphoid, myeloid, erythroid, and megakaryocyte progenitors, NK cells, germ cells, melanocytes, glial cells, vascular smooth muscle cells, placenta, and epithelial cells. Altered levels and mutations in CD117 are associated with several types of cancer including lung, breast, gastrointestinal stromal, and germ cell tumors.

Item	Value
Long Name	Stem Cell Factor Receptor
Entrez Gene IDs	3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names	CD117, CD117 antigen, EC 2.7.10, EC 2.7.10.1, KIT, PBT, Proto-oncogene c-Kit, SCF R, SCFR, SCFRmast/stem cell growth factor receptor, Tyrosine-protein kinase Kit, c-kit, ckit, piebald trait, proto-oncogene tyrosine-protein kinase Kit, soluble KIT variant 1, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Citations for Human CD117/c-kit Quantikine ELISA Kit (6)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD117/c-kit Quantikine ELISA Kit include:

Species: Human
Sample Types: Plasma

Necchi A et al. (2013-11-14), Analysis of plasma cytokines and angiogenic factors in patients with pretreated urothelial cancer receiving Pazopanib: the role of circulating interleukin-8 to enhance the prognostic accuracy. Br J Cancer, 2013-1110(1):26-33.
PMID: 24231947
Species: Human
Sample Types: Vitreous Humor

Abu El-Asrar et al. (2013-03-10), Angiogenic and vasculogenic factors in the vitreous from patients with proliferative diabetic retinopathy. J Diabetes Res, 2013-00(0):539658.
PMID: 23671874
Species: Human
Sample Types: Plasma

Llovet JM et al. (2012-02-28), Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma. Clin. Cancer Res., 2012-018(8):2290-300.
PMID: 22374331
Species: Human
Sample Types: Plasma

Zhu AX et al. (2009-05-26), Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study. J. Clin. Oncol., 2009-027(18):3027-35.
PMID: 19470923
Species: Human
Sample Types: Serum

Westerweel PE et al. (2008-09-28), Endothelial progenitor cell levels in obese men with the metabolic syndrome and the effect of simvastatin monotherapy vs. simvastatin/ezetimibe combination therapy. Eur. Heart J., 2008-029(22):2808-17.
PMID: 18824462
Species: Human
Sample Types: Plasma

Saltz LB et al. (2007-10-20), Phase II trial of sunitinib in patients with metastatic colorectal cancer after failure of standard therapy. J. Clin. Oncol., 2007-125(30):4793-9.
PMID: 17947727

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Product Documents for Human CD117/c-kit Quantikine ELISA Kit

Product Datasheets

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COA

SDS

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.