Human EGFR DuoSet ELISA

Name: Human EGFR DuoSet ELISA
Brand: R&D Systems
SKU: DY231
Rating: 5 (1 reviews)

Catalog # DY231 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (4)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	31.2 - 2,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human EGFR DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human EGFR DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human EGF R. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human EGFR DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human EGFR DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human EGFR DuoSet ELISA

	Human EGFR ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGFR

The EGF R subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular ligand binding domain containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites. The human EGF R cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain. Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse. Within the ECD, human EGF R shares 88% aa sequence identity with mouse and rat EGF R. It shares 43% - 44% aa sequence identity with the ECD of human ErbB2, ErbB3, and ErbB4. EGF R binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGF R is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs.

Item	Value
Long Name	Epidermal Growth Factor Receptor
Entrez Gene IDs	1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EGF R, EGFR, ERBB1PIG61, ErbB, ErbB1, HER-1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), mENA

Citations for Human EGFR DuoSet ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human EGFR DuoSet ELISA include:

Species: Human
Sample Types: Serum

A Harangu? et al. (2022-12-13), Identification of Potential microRNA Panels for Male Non-Small Cell Lung Cancer Identification Using Microarray Datasets and Bioinformatics Methods Journal of personalized medicine, 2022-112(12).
PMID: 36556276
Species: Human
Sample Types: Serum

Seyyed Mortaza Haghgoo (2016-12-05), Prognostic value of rare and complex mutations in EGFR and serum levels of soluble EGFR and its ligands in Iranian non-small cell lung carcinoma patients Clin. Biochem, 2016-10(0).
PMID: 27923629
Species: Human
Sample Types: Serum

Lemos-Gonzalez Y et al. (2007-04-24), Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma. Br. J. Cancer, 2007-096(10):1569-78.
PMID: 17453000
Species: Human
Sample Types: Cell Lysates

Konecny GE et al. (2006-02-01), Activity of the dual kinase inhibitor lapatinib (GW572016) against HER-2-overexpressing and trastuzumab-treated breast cancer cells. Cancer Res., 2006-066(3):1630-9.
PMID: 16452222

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Human EGFR DuoSet ELISA

Name: Anonymous

Sample Tested: EDTA Plasma

Verified Customer | Posted 11/08/2018

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Product Documents for Human EGFR DuoSet ELISA

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Human EGFR DuoSet ELISA

Catalog # DY231 | R&D Systems, Inc. a Bio-Techne Brand

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Summary for Human EGFR DuoSet ELISA

Product Specifications for Human EGFR DuoSet ELISA

Kit Contents for Human EGFR DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human EGFR DuoSet ELISA

Preparation and Storage

Background: EGFR

Citations for Human EGFR DuoSet ELISA (4)

Customer Reviews for Human EGFR DuoSet ELISA (1)

Human EGFR DuoSet ELISA

Product Documents for Human EGFR DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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