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Human Phospho-HGFR/c-MET DuoSet IC ELISA
Catalog # DYC2480-2 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Type: | Solid Phase Sandwich ELISA |
| Sample Types: | Cell Lysates |
| Human Phospho-HGFR/c-MET DuoSet IC ELISA Features: |
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Figure 1. The Human Phospho-HGF R DuoSet IC ELISA is more sensitive than immunoprecipitation (IP)-Western analysis The human epidermoid carcinoma cell line, A431, was treated with 100 ng/mL recombinant human HGF for five minutes to induce tyrosine phosphorylation of HGF R. Serial dilutions of lysates were analyzed by (A) IP-Western blot and (B) this ELISA. IPs were done using an anti-HGF R polyclonal antibody and Protein G agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-HGF R (p-HGF R). Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (Catalog # AR004). |
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Figure 2. The Human Phospho-HGF RDuoSet IC ELISA detects ligand-induced HGF R tyrosine phosphorylation A431 cells were untreated or treated with 100 ng/mL recombinant human HGF for five minutes. ELISA and IP-Western blot (inset) analyses were done using 50 μg and 500 μg of lysate, respectively. IP-Western blots for phospho-HGF R (p-HGF R) were done as described in Figure 1. Blots were stripped and total HGF R was detected using a biotinylated anti-HGF R polyclonal antibody (Catalog # BAF358). |
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Figure 3. The specificity of the Human Phospho-HGF RDuoSet IC ELISA is confirmed by receptor competition A431 cells were treated with 100 ng/mL recombinant human HGF for five minutes. The indicated amounts of recombinant extracellular domains of human HGF R (Catalog #358-MT), human EGF R (Catalog #1095-ER) or human IGF-I sR (Catalog #305-GR) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant HGF R. |
Citations for Human Phospho-HGFR/c-MET DuoSet IC ELISA (5)
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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-HGFR/c-MET DuoSet IC ELISA include:
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Species: Human
Sample Types: Cell Culture Supernates
M Su et al. (2017-11-23), Andrographolide enhanced 5-fluorouracil-induced antitumor effect in colorectal cancer via inhibition of c-MET pathway Drug Des Devel Ther, 2017-111(0):3333-3341.
PMID: 29200829 -
Species: Human
Sample Types: Cell Culture Supernates
Fu et al. (2013-03-01), Shedding of c-Met ectodomain correlates with c-Met expression in non-small cell lung cancer. Biomarkers, 2013-018(2):126-35.
PMID: 23410046 -
Species: Human
Sample Types: Cell Lysates
Xu L et al. (2010-02-15), Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells. Oncogene, 2010-029(18):2616-27.
PMID: 20154724 -
Species: Human
Sample Types: Cell Lysates
Uhland K et al. (2009-08-01), Use of IHC and newly designed matriptase inhibitors to elucidate the role of matriptase in pancreatic ductal adenocarcinoma. Int. J. Oncol., 2009-035(2):347-57.
PMID: 19578749 -
Species: Human
Sample Types: Cell Lysates
Phin S et al. (2009-05-15), Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice. Am. J. Respir. Cell Mol. Biol., 2009-042(3):286-93.
PMID: 19448157
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Customer Reviews for Human Phospho-HGFR/c-MET DuoSet IC ELISA (1)
FAQs for Human Phospho-HGFR/c-MET DuoSet IC ELISA
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Which phosphorylated sites are recognized by this assay?
This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
