Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF Best Seller

Name: Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF
Brand: R&D Systems
SKU: 305-GR

Catalog # 305-GR | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	P08069
Applications:	Binding Activity

View Full Technical Details for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

Technical Data
Citations (2) / Reviews

Product Specifications for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

Source	Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein Met1-Asn932 with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu31 and Asp741
Predicted Molecular Mass	104 kDa (single chain), 81 kDa ( alpha subunit) and 23 kDa ( beta subunit)
SDS-PAGE	104 kDa (single chain), 81 kDa ( alpha subunit) and 23 kDa ( beta subunit)
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human IGF‑I R/IGF1R is present at 0.5 μg/mL, the concentration of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) that produces 50% of the optimal binding response is found to be approximately 2‑10 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

305-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl, EDTA and Tween®-20 with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

Item	Value
References	Rechler, M.M. and S.P. Nissley (1990) Insulin-Like Growth Factors, in Peptide Growth Factors and Their Receptors I, M.B. Sporn and A.B. Roberts, editors, Springer-Verlag, New York, New York, p. 263.
Long Name	Insulin-like Growth Factor I Receptor
Entrez Gene IDs	3480 (Human)
Alternate Names	CD221, CD221 antigen, EC 2.7.10, EC 2.7.10.1, IGF-1R, IGF-I R, IGF-I receptor, IGF-IR, IGF1R, IGFIR, IGFR, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216, insulin-like growth factor 1 receptor, soluble IGF1R variant 1, soluble IGF1R variant 2

Citations for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF include:

Species: Yeast
Sample Types: Whole Cells
Applications: Bioassay

Xu et al. (2013-02-07), Rapid optimization and prototyping for therapeutic antibody-like molecules. MAbs, 2013-05(2):237-54.
PMID: 23392215
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Iozzo RV et al. (2011-08-12), Decorin antagonizes IGF receptor I (IGF-IR) function by interfering with IGF-IR activity and attenuating downstream signaling. J. Biol. Chem., 2011-0286(40):34712-21.
PMID: 21840990

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Product Documents for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

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