Human HGFR/c‑MET Biotinylated Antibody
Catalog # BAF358 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human HGFR/c‑MET Biotinylated Antibody
Immunogen
Glu25-Thr932
Accession # P08581
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human HGFR/c‑MET Biotinylated Antibody
Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Human HGF R/c-MET Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF358, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).Applications for Human HGFR/c‑MET Biotinylated Antibody
Flow Cytometry
Sample: MDA‑MB‑231 human breast cancer cell line
Western Blot
Sample: Recombinant Human HGF R/c-MET Fc Chimera (Catalog # 358-MT)
Human HGF R/c-MET Sandwich Immunoassay
Reviewed Applications
Read 1 review rated 4 using BAF358 in the following applications:
Published Applications
Read 6 publications using BAF358 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGFR/c-MET
HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta-propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha6/ beta4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% aa sequence identity with canine, mouse, and rat HGF R.
References
- Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
- Corso, S. et al. (2005) Trends Mol. Med. 11:284.
- Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
- Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
- Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
- Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
- Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
- Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
- Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
- Ponzetto, C. et al. (1994) Cell 77:261.
- Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
- Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
- Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
- Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
- Wang, X. et al. (2002) Mol. Cell 9:411.
- Trusolino, L. et al. (2001) Cell 107:643.
- Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
- Conrotto, P. et al. (2004) Oncogene 23:5131.
- Follenzi, A. et al. (2000) Oncogene 19:3041.
- Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
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UniProt
Product Documents for Human HGFR/c‑MET Biotinylated Antibody
Product Specific Notices for Human HGFR/c‑MET Biotinylated Antibody
For research use only
Citations for Human HGFR/c‑MET Biotinylated Antibody (6)
Citations are publications that use Bio-Techne products. Selected citations for Human HGFR/c‑MET Biotinylated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: ICCW Li et al. (2019-02-13), Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering Sci Rep, 2019-09(1):1988.
PMID: 30760737 -
Species: Human
Sample Types: Whole Tissue
Applications: ELISA Development (Capture)Deborah R Kaye (2016-06-14), Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer PLoS ONE, 2016-011(6):e0157130.
PMID: 27300295 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotCen B et al. (2014-04-28), The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling. Mol Cell Biol, 2014-034(13):2517-32.
PMID: 24777602 -
Species: Human
Sample Types: Serum
Applications: ELISA Development (Capture)Tabernero J et al. (2014-03-14), A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases. Clin Cancer Res, 2014-020(10):2793-804.
PMID: 24634378 -
Species: Human
Sample Types: Plasma
Applications: Electrochemiluminescent AssayGordon MS et al. (2010-01-12), Safety, pharmacokinetics, and pharmacodynamics of AMG 102, a fully human hepatocyte growth factor-neutralizing monoclonal antibody, in a first-in-human study of patients with advanced solid tumors. Clin. Cancer Res., 2010-016(2):699-710.
PMID: 20068101 -
Species: Human
Sample Types: Cell Lysates
Applications: Electrochemiluminescent AssayCoxon A et al. (2009-05-12), Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models. Mol. Cancer Ther., 2009-00(0).
PMID: 19435874
There are no citations that match your criteria.
Customer Reviews for Human HGFR/c‑MET Biotinylated Antibody (1)
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