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T Cell Manufacturing

T cells exert multiple functions that are critical for immune responses, and these properties can be harnessed and redirected by a variety of immunotherapy strategies. CAR-T cells and TCR transgenic T cells are designed to target specific tumor antigens and to respond with anti-tumor activities. This may be cytotoxic activity directed at the tumor cells, secretion of cytokines that support local immune responses, or development of long-term anti-tumor immunity.

Learn more below about our T cell manufacturing capabilities or contact our Custom Services team for requests tailored exactly to what you need.

Simplified Solutions for T Cells from Development to Commercialization

Isolation and Cell Culture for T Cells

Isolation and Cell Culture for T Cells

Expand T cell cultures from PBMCs or purified CD3+ PBMCs with our ProDots™ proteins, ExCellerate™ media, and Cloudz™ cell activation kit. These culture tools are optimized for use with each other. We offer directly comparable RUO and GMP grades for seamless transition from research to manufacturing.

Cell Selection

Bioactive Cytokines

  • GMP ProDots proteins are formulated as dissolvable spheres enclosed inside sterile, weldable bags for closed processing cytokine addition.
  • Animal-free GMP proteins are produced in dedicated controlled-access laboratories to prevent potential contamination by animal components or byproducts.

Small Molecules

Cell Culture Media

 

Cell Activation Kits

T Cell Expansion and Stimulation

Expansion of Human T Cells using Cloudz™ Human T Cell Activation Kit

T cells were expanded from primary human PBMCs with the GMP Cloudz T cell kit along with ExCellerate T cell media and human IL-2 protein.

ProDots Bag

GMP ProDots Proteins are pre-aliquoted, lyophilized, and instantly dissolvable spheres of animal-free GMP cytokines or growth factors. They are conveniently packaged into single-use bags for easy incorporation into closed-system processes. Custom created options can be developed to precisely fit into a specific system.

Genome Engineering for T Cells

Genome Engineering

We offer solutions for both non-viral and virus-based gene editing. Our TcBusterTM technology, powered by R&D Systems, is a non-viral transposon system that provides stable integration of your gene of interest (GOI) into any cell type. Our in-house Process Development Services can streamline any gene modified workflow.

TcBuster Gene Transfer System

  • Improved integration rates compared to traditional methods
  • Rapid generation of transgenic mammalian cells with stable transgene expression
  • Use is not restricted by licensing
  • Does not provoke anti-viral immune responses

 

Contract Development and Manufacturing at R&D Systems

  • ISO7 cleanrooms for manufacturing GMP-grade engineered cells or processing Phase I/II trial samples
  • Advance to clinical testing in 4 months
  • Learn more about GMP CDMO Process Development Services 

Vector Characterization with Instruments by ProteinSimple

 

Learn how our TcBuster technology can assist with you with Therapeutic Genome Engineering using T cells.

See the Data: CAR Insertion Into Primary T Cells

CD19 CAR T Cells

Insertion of a CD19 CAR into primary human T cells. The integration efficiency with transposition is slightly higher than with transduction.

Graph of transposed CAR-T cytotoxicity

Gene edited cells were incubated with CD19+ NALM6 cells. Only CD19 CAR-expressing cells were able to kill the NALM6 cells. Both transposed and transduced cells had similar killing efficiency.

T Cell Characterization Using Various Bio-Techne Brands

T Cell Characterization

Characterize your cells at every manufacturing stage to ensure a robust process. Confirm that they exhibit the correct phenotype and their purity meets your requirements. Our extensive selection of premium reagents and instruments allows you to quickly analyze cell surfaces and secretory profiles and to screen for residual contaminants.

Cell Phenotype

 

Cell Function and Potency

Contaminant Screening

 

Tissue Biopsy and T Cell Localization

  • GCLP-compliant RNAscopeTM ISH analysis for clinical and preclinical studies

T Cell Analysis

Example of T cell Activation

Confirmation of chimeric antigen receptor expression. Human CD4+ CD8+ T cells were transduced with a CD19-CAR (left) or not transduced (right). Cells were stained with recombinant human CD19 Fc Chimera Atto 488 protein to detect chimeric antigen receptor expression by flow cytometry.

T Cell Analysis

Cell culture analysis with Micro-Flow Imaging (MFI) discriminates between T cells and residual activation beads based on equivalent circular diameter. The inset shows images of particulates in the sample as captured by MFI. The histogram shows resolution of live cells from activation beads and the ability of MFI to quantitate the number of beads remaining in the sample.

Find Antibodies For T Cell Identification

Explore our interactive cell marker tool to view the cell surface and intracellular markers most used to distinguish different CD4+ helper T cells. All markers are linked to R&D SystemsTM antibodies to make finding your antibody easy.

Visit R&D Systems to Explore Our Interactive Cell Marker Tool

Custom Services

Our custom service team is committed to developing optimized solutions to streamline your T cell manufacturing processes. We will work with you to develop and deliver reagents, culture media, and immunoassays that meet your requirements.

  • Our Cloudz cell activation technology by R&D Systems can be customized with a breadth of ligands to drive your specific T cell expansion process.
  • Our GMP ProDots proteins can be custom packaged for your required cytokine mass in each sterile bag.
  • Our R&D Systems Custom Antibody Services can optimize your cell selection and phenotyping with GMP antibodies, recombinant antibody engineering, and monoclonal panel development.

 

For inquiries regarding specialized T cell services, speak with an expert at Custom Services for Cell & Gene Therapies.

ScaleReady Logo

Bio-Techne believes in the power of partnerships. We have joined with Fresenius Kabi and Wilson Wolf to bring you ScaleReady™ for immune cell therapy manufacturing.

ScaleReady brings cell and gene therapies to life by streamlining manufacturing processes across cell culture, cell activation and expansion, gene editing, and cell processing. 

Learn More at ScaleReady.com

Background Information

Luminex is a registered trademark of Luminex Corporation