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Human Magnetic Luminex Assay

Luminex Discovery Assays

Luminex Discovery Assays provide the most flexible assays on the market. These panels are completely customizable using our large menu of human, mouse, and rat analytes, making them the perfect tool for biomarker discovery and screening.

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Key Product Details

Assay Type: Magnetic bead-based multiplex assay for the Luminex® platform
Format: 1 x 96-well microplate and magnetic antibody-coated beads
Species: Human
Analytes Detected: Select from over 350 analytes to build your custom panel using our assay customization tool below.

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Citations (188)

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Customer Reviews (31)

4.5 out of 5
31 customer ratings
5 stars
65%
4 stars
26%
3 stars
6%
2 stars
0%
1 star
3%

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  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 05/04/2021
    First time using these kits - analysed 25 analytes. Most had a r^2 on standard curve of >0.999. One (IGFBP3) frequently ended up being 0.97, with % recovery frequently outside the 80-120% range. Spoke to R&D who were very helpful, provided significant technical support, and ultimately ended up testing their remnants of the kit that they had left over, confirming an error with the kit for IGFBP3.Used the R&D guarantee, and will report back on the quality of the replacement kits.
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Sputum sol
    Verified Customer | Posted 02/09/2021
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 11/17/2020
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/04/2020
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Cell Culture Supernates
    Verified Customer | Posted 10/01/2020
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Verified Customer | Posted 06/05/2020
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: A375 cell supernatants
    Verified Customer | Posted 04/27/2020
    First time I ever used the Magnetic Luminex Assay and I had no trouble running the assay.
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 04/21/2020
    a target protein expressing cancer cells co-incubate with PBMC
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 04/21/2020
    cancer cell co-incubate with healthy donor PBMC with lead candidate mAbs.
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 02/25/2020
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: in vitro cell culture
    Verified Customer | Posted 10/19/2019
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: in vitro cell culture
    Verified Customer | Posted 08/13/2019
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 06/19/2019
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Saliva
    Verified Customer | Posted 04/02/2019
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: HL-60 human acute promyelocytic leukemia cell line
    Verified Customer | Posted 01/30/2019
    Gave us clear readouts of the level of RANTES in our samples
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Electrospun Templates,Manuka Honey,Neutrophils
    Verified Customer | Posted 01/17/2019
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 01/16/2019
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: mouse serum and recombinant TNF alpha
    Verified Customer | Posted 01/07/2019
    the standard was great but the test did not detect TNF alpha in our material while other ELISAs did
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 07/13/2018
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 07/13/2018
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Heparin Plasma
    Verified Customer | Posted 05/28/2018
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 05/28/2018
    We found that the volumes for beads, antibodies, and streptavidin biotin were higher than in previous kits. Even a couple of mls more made easier to prepare the suspension with enough volume to run a whole plate. Bubbles usually make the experiment more complicated but this time we did not find that the buffers were too foamy.
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Heparin Plasma
    Verified Customer | Posted 05/13/2018
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 04/03/2018
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Human tissue
    Verified Customer | Posted 12/15/2017
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: LXSAHM
    Verified Customer | Posted 11/13/2017
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: induced sputum
    Verified Customer | Posted 10/27/2017
    This was the first time we ran induced sputums to test these biomarkers. The values obtained were low or negative. We used the samples without dilution as we had no literature data to refer to.
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Heparin Plasma
    Verified Customer | Posted 10/27/2017
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Heparin Plasma,induced sputum
    Verified Customer | Posted 10/27/2017
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Tissue Culture Media
    Verified Customer | Posted 06/14/2016
    Human Magnetic Luminex Assay Serum LXSAHM
  • Human Magnetic Luminex Assay
    Name: Anonymous
    Sample Tested: Tissue Culture Media
    Verified Customer | Posted 06/13/2016
    Human Magnetic Luminex Assay Serum LXSAHM

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FAQs

  • What is the reason for the recommendation to use 35 µL sample volume, instead of the default 50 µL volume, for Luminex multiplex containing > 25 analytes, when assayed on Luminex MAGPIX® analyzer?

    R&D Systems recommend 35 µL sample volume for > 25 analytes to control the number of beads in the sample load when using Luminex MAGPIX® analyzer. Luminex MAGPIX® analyzer is a non-flow-based reader which utilizes a magnet to hold the magnetic microparticles in a monolayer in a chamber where the beads are imaged and then processed by the software to generate data. Since MAGPIX® system pushes the entire Sample Load Volume into the chamber, multiplex assay containing > 25 analytes may overload the chamber capacity with 50 µL sample load default setting. The captured images can have too many beads in them to give accurate results, and some of the analytes/regions may present lower than desired bead counts. 

Product Datasheets

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