Human IFN-gamma Quantikine HS ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # HSDIF0
Key Product Details
Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (0 Not Assigned), Heparin Plasma (100 µL)
Sensitivity
0.173 pg/mL
Assay Range
0.469-30 pg/mL (Serum, Plasma, EDTA Plasma, Heparin Plasma)
Product Summary for Human IFN-gamma Quantikine HS ELISA Kit
The Quantikine® HS Human IFN-gamma Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IFN-gamma in serum and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IFN-gamma.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
EDTA Plasma, Heparin Plasma, Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.83 | 6.10 | 12.8 | 2.15 | 6.33 | 12.4 |
| Standard Deviation | 0.084 | 0.187 | 0.253 | 0.210 | 0.421 | 0.898 |
| CV% | 4.6 | 3.1 | 2.0 | 9.8 | 6.7 | 7.2 |
Recovery for Human IFN-gamma Quantikine HS ELISA Kit
The recovery of human IFN-gamma spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 99 | 90-108 |
| Heparin Plasma (n=4) | 99 | 92-109 |
| Serum (n=4) | 97 | 85-109 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human IFN-gamma were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IFN-gamma Quantikine HS ELISA Kit
Human IFN-gamma HS Quantikine ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Long Name
Interferon gamma
Alternate Names
IFG, IFI, IFNG, IFNgamma
Entrez Gene IDs
Gene Symbol
IFNG
Additional IFN-gamma Products
Product Documents for Human IFN-gamma Quantikine HS ELISA Kit
Product Specific Notices for Human IFN-gamma Quantikine HS ELISA Kit
For research use only
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