Human/Mouse IL-12 R beta 2 PE-conjugated Antibody

Name: Human/Mouse IL-12 R beta 2 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB1959P

Catalog # FAB1959P

Citations (3)

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2 Cys28-Asn622 Accession # Q99665
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 305719
Specificity:	Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed.

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Applications

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse IL-12 R beta 2 PE-conjugated Antibody Scientific Data Examples

	Flow Cytometry Detection of IL‑12 R beta 2 in Human TH1 cells by Flow Cytometry. Human CD4⁺peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL plate-bound Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100), 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL), and 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 5 days to induce Th1 cell development, followed by restimulation with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 2 to 3 hours, were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and Mouse Anti-Human/Mouse IL-12 R beta 2 PE-conjugated Monoclonal Antibody (Catalog # FAB1959P). Quadrant markers were set based on staining using Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: IL-12 R beta 2

Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma. It is also required for the expansion and activation Th1 cells (1, 2).

The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL‑12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) protein with a putative 27 aa signal peptide that is cleaved to generate the mature protein with a 595 aa extracellular domain, a 24 aa transmembrane domain and a 216 aa cytoplasmic region. Human and mouse IL‑12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL‑12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).

Item	Value
References	Trinchieri, G. et al. (2003) Immunity 19:641. Brombacher, F. et al. (2003) Trends in Immunol. 23:207. Trinchieri, G. (2003) Nature Reviews Immunol. 3:133. Rogge, L. et al. (1997) J. Exp. Med. 185:825.
Long Name	Interleukin 12 Receptor beta 2
Entrez Gene IDs	3595 (Human); 16162 (Mouse)
Alternate Names	IL-12 R beta 2, IL-12 receptor beta 2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12Rb2, IL12R beta 2, IL12RB2, interleukin 12 receptor, beta 2, interleukin-12 receptor beta-2 chain, interleukin-12 receptor subunit beta-2

Citations for Human/Mouse IL-12 R beta 2 PE-conjugated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse IL-12 R beta 2 PE-conjugated Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

B Baban et al. (2021), AMPK induces regulatory innate lymphoid cells after traumatic brain injury JCI Insight, 6(1).
PMID: 33427206
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

CA Cunningham et al. (2016), POSH Regulates CD4+ T Cell Differentiation and Survival J Immunol, 0(0).
PMID: 27084103
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Freeman B et al. (2014), Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions. J Virol, 88(13):7474-84.
PMID: 24741101

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