Human/Mouse IL-12 R beta 2 Alexa Fluor® 488-conjugated Antibody

Name: Human/Mouse IL-12 R beta 2 Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: FAB1959G

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB1959G

Citations (3)

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2 Cys28-Asn622 Accession # Q99665
Applications:	Flow Cytometry
Label:	Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 305719
Specificity:	Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed.

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Applications

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples

	Flow Cytometry Detection of IL‑12 R beta 2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) and 10 µg/mLGoat Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse IL-12 R beta 2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1959G) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: IL-12 R beta 2

Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma. It is also required for the expansion and activation Th1 cells (1, 2).

The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL‑12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) protein with a putative 27 aa signal peptide that is cleaved to generate the mature protein with a 595 aa extracellular domain, a 24 aa transmembrane domain and a 216 aa cytoplasmic region. Human and mouse IL‑12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL‑12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).

Item	Value
References	Trinchieri, G. et al. (2003) Immunity 19:641. Brombacher, F. et al. (2003) Trends in Immunol. 23:207. Trinchieri, G. (2003) Nature Reviews Immunol. 3:133. Rogge, L. et al. (1997) J. Exp. Med. 185:825.
Long Name	Interleukin 12 Receptor beta 2
Entrez Gene IDs	3595 (Human); 16162 (Mouse)
Alternate Names	IL-12 R beta 2, IL-12 receptor beta 2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12Rb2, IL12R beta 2, IL12RB2, interleukin 12 receptor, beta 2, interleukin-12 receptor beta-2 chain, interleukin-12 receptor subunit beta-2

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human/Mouse IL-12 R beta 2 Alexa Fluor® 488-conjugated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse IL-12 R beta 2 Alexa Fluor® 488-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

R Sun et al. (2020), IL23 Promotes Antimicrobial Pathways in Human Macrophages, Which Are Reduced With the IBD-Protective IL23R R381Q Variant Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32474165
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

JD Coakley et al. (2020), Innate Lymphocyte Th1 and Th17 Responses in Elderly Hospitalised Patients with Infection and Sepsis Vaccines (Basel), 8(2).
PMID: 32560376
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

K Kachler et al. (2017), Enhanced acid sphingomyelinase activity drives immune evasion and tumor growth in non-small cell lung carcinoma Cancer Res., 0(0).
PMID: 28883000

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