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Recombinant Human IL-12 Protein

R&D Systems, Inc. a Bio-Techne Brand

HEK293-expressed Version Available!
Recombinant Human IL-12 Protein Bioactivity
(2)
Recombinant Human IL-12 Protein SDS-PAGE
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219-IL-005
219-IL-025

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Carrier Free
219-IL-025/CF
219-IL-005/CF
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Key Product Details

Source: Sf 21 (baculovirus)
Accession #: P29460 (p40) & P29459 (p35)
Structure / Form: Disulfide-linked heterodimer
Applications: Bioactivity

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Citations for Recombinant Human IL-12 Protein (117)

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Customer Reviews for Recombinant Human IL-12 Protein (7)

4.6 out of 5
7 customer ratings
5 stars
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  • Recombinant Human IL-12 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 05/13/2021
    Recombinant Human IL-12 Protein Immunoassay Standard 219-IL
  • Recombinant Human IL-12 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 07/07/2020
    Recombinant Human IL-12 Protein Immunoassay Standard 219-IL
  • Recombinant Human IL-12 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/08/2020
  • Recombinant Human IL-12 Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 03/19/2019
  • Recombinant Human IL-12 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/08/2018
  • Recombinant Human IL-12 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 07/27/2018
  • Recombinant Human IL-12 Protein
    Name: Anonymous
    Application: In vivo study
    Reason for Rating: Perform cytokines measurements for different T cells
    Verified Customer | Posted 03/26/2018

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FAQs for Recombinant Human IL-12 Protein

  • What is the difference between Recombinant Human IL-12 Protein (Catalog # 219-IL) and Recombinant Human Il-12 (linked heterodimer) Protein (Catalog # 10018-IL?

    The Recombinant Human IL-12 Protein (Catalog # 219-IL), expressed in Sf 21 cells, is a disulfide-linked homodimer consisting of the p35 and p40 subunits. The subunits can be separated under reducing conditions. The Recombinant Human IL-12 (linked heterodimer) Protein (Catalog # 10018-IL), expressed in HEK293 cells, is a chimeric protein consisting of the p35 and p40 subunits connected by a linker. These two subunits cannot be separated.

  • Human IL-23 and Human IL-12 share a p40 subunit.  Why is the predicted molecular weight for the p40 subunit 36.5 kDa in the Recombinant Human IL-23 Protein (Catalog # 1290-IL), while the predicted molecular weight for the p40 subunit is 34.7 kDa for the Recombinant Human IL-12 Protein  (Catalog # 219-IL)?

    The Recombinant Human IL-23 Protein (Catalog # 1290-IL) and Recombinant Human IL-12 Protein (Catalog # 219-IL) p40 subunits share the same p40 sequence, Ile23-Ser328 (Accession # P29460).  However, the p40 subunit for Recombinant Human IL-23 (Catalog # 1290-IL) also contains a portion of the GS linker that remains after cleaving p40 subunit from the p19 subunit, so its predicted molecular is slightly larger (36.5 kDa) compared to that of the Recombinant Human IL-12 Protein (Catalog # 219-IL; 34.7 kDa).  Please see the product-specific page for detailed sequence information.

Product Documents for Recombinant Human IL-12 Protein

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