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Recombinant Human IL-12 (linked heterodimer) Protein, CF Best Seller

Catalog # 10018-IL | R&D Systems, Inc. a Bio-Techne Brand
GS Linker, Defined heterodimer stoichiometry.
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10018-IL-010
10018-IL-020
10018-IL-050
10018-IL-100

Key Product Details

Source

HEK293

Structure / Form

GS-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-12 protein
Human IL-12p40
(Ile23-Ser328)
Accession # P29460.1
GSGSSRGGSGSGGSGGGGSK Human IL-12p35
(Arg23-Ser219)
Accession # P29459.2
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

59 kDa

SDS-PAGE

63-75 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is 0.01-0.05 ng/mL.

Scientific Data Images for Recombinant Human IL-12 (linked heterodimer) Protein, CF

Recombinant Human IL-12 (linked heterodimer) Protein Bioactivity

Recombinant Human IL-12 (linked heterodimer) Protein Bioactivity

Recombinant Human IL-12 (Catalog # 10018-IL) stimulates proliferation in PHA-activated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL.
Recombinant Human IL-12 (linked heterodimer) Protein SDS-PAGE

Recombinant Human IL-12 (linked heterodimer) Protein SDS-PAGE

2 μg/lane of Recombinant Human IL‑12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63-75 kDa.

Formulation, Preparation and Storage

10018-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100-200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines (1). The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF (2, 3). These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells (4). These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN‑gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells (5). Current evidence indicates that IL‑12, produced by macrophages in response to infectious agents, is a central mediator of the cell‑mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

References

  1. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
  2. Gearing, D. et al. (1991) Cell 66:9.
  3. Merberg, D. et al. (1992) Immunology Today 13:78.
  4. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.
  5. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Gene Symbol

IL12A

Additional IL-12 Products

Product Documents for Recombinant Human IL-12 (linked heterodimer) Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-12 (linked heterodimer) Protein, CF

For research use only

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FAQs for Recombinant Human IL-12 (linked heterodimer) Protein, CF

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  • Q: What is the difference between Recombinant Human IL-12 Protein (Catalog # 219-IL) and Recombinant Human Il-12 (linked heterodimer) Protein (Catalog # 10018-IL?

    A: The Recombinant Human IL-12 Protein (Catalog # 219-IL), expressed in Sf 21 cells, is a disulfide-linked homodimer consisting of the p35 and p40 subunits. The subunits can be separated under reducing conditions. The Recombinant Human IL-12 (linked heterodimer) Protein (Catalog # 10018-IL), expressed in HEK293 cells, is a chimeric protein consisting of the p35 and p40 subunits connected by a linker. These two subunits cannot be separated.

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