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Key Product Details

Species Reactivity

Human

Applications

CyTOF-reported, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, T Cell Stimulation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # UCHT1

Product Summary for Human CD3 epsilon Antibody

Immunogen

Human thymocytes (1)

Specificity

Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD3 epsilon Antibody

CD3e antibody in Human PBMCs by Immunocytochemistry (ICC).

CD3 epsilon in Human PBMCs.

CD3e was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human CD3 epsilon by Western Blot.

Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and THP‑1 human acute monocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of CD3e antibody in Human Lymphocytes antibody by Flow Cytometry.

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human CD3 epsilon Antibody

Application
Recommended Usage

CyTOF-reported

Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunoprecipitation

McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York

T Cell Stimulation

This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).

Western Blot

2 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using MAB100 in the following applications:

Published Applications

Read 57 publications using MAB100 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References

  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for Human CD3 epsilon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD3 epsilon Antibody

For research use only

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FAQs for Human CD3 epsilon Antibody

Showing  1 - 2 of 2 FAQs Showing All
  • Q: What is the protocol used to immobilize MAB100 for T cell stimulation?What is the protocol used to immobilize MAB100 for T cell stimulation?

    A: The protocol is based on the following reference: J. Immunology. (1992) 149:3448-3455. 

  • Q: What is the optimal working concentration of the antibody?

    A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.

Showing  1 - 2 of 2 FAQs Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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