Human CD3 epsilon Antibody

Name: Human CD3 epsilon Antibody
Brand: R&D Systems
SKU: MAB100
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB100

Citations (56)

(4)

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Clone UCHT1 was used by HLDA to establish CD designation

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human thymocytes (1)
Applications:	CyTOF-reported, Flow Cytometry, Functional Assay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # UCHT1
Specificity:	Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

View Full Technical Details for Human CD3 epsilon Antibody

Applications for Human CD3 epsilon Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Flow Cytometry	0.25 µg/10⁶ cells	See below
Immunoprecipitation	McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York
CyTOF-reported	Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
T Cell Stimulation	This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD3 epsilon Antibody

	Western Blot Detection of Human CD3 epsilon by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and THP‑1 human acute monocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Immunocytochemistry CD3 epsilon in Human PBMCs. CD3e was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Flow Cytometry Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Item	Value
References	Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs	916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names	CD3 epsilon, CD3-epsilon, CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), FLJ18683, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE

Citations for Human CD3 epsilon Antibody (55)

Showing 1 - 10 of 55 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CD3 epsilon Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Yang et al. (2022), Overexpression of ascitic interleukin-35 induces CD8+ T cell exhaustion in liver cirrhotic patients with spontaneous bacterial peritonitis International immunopharmacology, 108(0):108729.
PMID: 35349961
Species: Human
Sample Types: Whole Cells
Applications: Stimulation

D Peruzzu et al. (2022), Anti-Inflammatory Effects of 1,25(OH)2D/Calcitriol in T Cell Immunity: Does Sex Make a Difference? International Journal of Molecular Sciences, 23(16).
PMID: 36012424
Species: Primate
Sample Types: Whole Tissue
Applications: IHC

RM Gopalakris et al. (2021), Increased IL-6 expression precedes reliable viral detection in the rhesus macaque brain during acute SIV infection JCI Insight, 6(20).
PMID: 34676832
Species: Human
Sample Types: Whole Cells
Applications: T Cell Activation

J Huetter et al. (2020), Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2) Cancer Immunol Res, 8(7):895-911.
PMID: 32312711
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development

MC Cavé et al. (2020), Glycosaminoglycans bind human IL-27 and regulate its activity Eur. J. Immunol., 0(0).
PMID: 32483835
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

N Beha et al. (2019), IL-15-based trifunctional antibody-fusion proteins with costimulatory TNF-superfamily ligands in the single-chain format for cancer immunotherapy Mol. Cancer Ther., 0(0).
PMID: 31040163
Species: Human
Sample Types: Whole Cells
Applications: Stimulation

NB Zhou et al. (2019), Effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain in vitro Oncol Lett, 18(4):4230-4236.
PMID: 31516618
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

JC Chamcheu et al. (2019), Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 8(9).
PMID: 31540162
Species: Human
Sample Types: Whole Tissue
Applications: Tissue Culture

ML Tiirikaine et al. (2019), Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 0(0).
PMID: 31948839
Species: Human
Sample Types: Whole Cells
Applications: Stimulation

MA Dragovich et al. (2019), SLAMF6 clustering is required to augment T cell activation PLoS ONE, 14(6):e0218109.
PMID: 31199820
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Bensch et al. (2018), 89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer Nat. Med., 0(0).
PMID: 30478423
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Strazza et al. (2017), PLC?1 regulates SDF-1?-induced lymphocyte adhesion and migration to sites of inflammation Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28213494
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Tao et al. (2017), ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function Mol Med Rep, 0(0).
PMID: 28259946
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

S Nakachi et al. (2017), Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 19(1):97.
PMID: 28511719
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

R Fischer et al. (2017), Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2 Sci Rep, 7(1):6607.
PMID: 28747780
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

C Caër et al. (2017), Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue Sci Rep, 7(1):3000.
PMID: 28592801
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Elena Neumann (2016), Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts Matrix Biol., 0(0).
PMID: 27884778
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Xun Qu (2016), Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2 Cell. Mol. Immunol., 13(5):615-27.
PMID: 26027727
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Patsoukis N et al. (2015), PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation. Nat Commun, 6(0):6692.
PMID: 25809635
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Huang W et al. (2015), T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines. J Exp Clin Cancer Res, 34(0):54.
PMID: 25989794
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Moore C et al. (2015), Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation. J Immunol, 194(2):761-72.
PMID: 25505283
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Todaro M et al. (2014), CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis. Cell Stem Cell, 14(3):342-56.
PMID: 24607406
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Cevikbas F et al. (2014), A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1. J Allergy Clin Immunol, 133(2):448-60.
PMID: 24373353
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Gao D et al. (2014), Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in gastric and colorectal cancer patients. PLoS ONE, 9(4):e93886.
PMID: 24699863
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhu L et al. (2014), Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. J Thromb Haemost, 12(7):1156-65.
PMID: 24833264
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Sgarbanti M et al. (2014), IkappaB kinase epsilon targets interferon regulatory factor 1 in activated T lymphocytes. Mol Cell Biol, 34(6):1054-65.
PMID: 24396068
Van Belle T et al. (2014), 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients. PLoS ONE, 9(10):e109194.
PMID: 25279717
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Ruan M et al. (2014), The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma. Int J Oncol, 44(6):2103-10.
PMID: 24676671
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Fan et al. (2014), Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif. PLoS ONE, 9(9):e108253.
PMID: 25250958
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Wang et al. (2013), Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases. J Biol Chem, 288(41):29223-8.
PMID: 24003230
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Renand A et al. (2013), Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs. PLoS ONE, 8(12):e85589.
PMID: 24386482
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Ramgolam VS et al. (2011), B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis. J. Immunol., 186(0):4518.
PMID: 21368231
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jones GW et al. (2011), Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. FASEB J., 25(1):409-19.
PMID: 20826539
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Pennino D et al. (2010), IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. J. Immunol., 184(9):4880-8.
PMID: 20357258
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Eikawa S et al. (2010), Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6. J. Immunol., 185(11):6734-40.
PMID: 21048114
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Ni Z et al. (2009), Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation. Clin. Immunol., 133(2):257-64.
PMID: 19665434
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Witte K et al. (2009), Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines. Genes Immun., 10(8):702-14.
PMID: 19798076
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Lundqvist A et al. (2009), Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 113(24):6120-7.
PMID: 19202127
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Hirosako S et al. (2009), Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin. Exp. Immunol., 155(2):266-74.
PMID: 19040600
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Longo O et al. (2009), Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up. Vaccine, 27(25):3306-12.
PMID: 19208456
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Tran CN et al. (2008), Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3. J. Immunol., 180(5):2989-98.
PMID: 18292521
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Rossol M et al. (2007), Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells. J. Immunol., 179(6):4239-48.
PMID: 17785864
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Bayry J et al. (2007), Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells. J. Immunol., 178(7):4184-93.
PMID: 17371975
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

McFadden C et al. (2007), Preferential migration of T regulatory cells induced by IL-16. J. Immunol., 179(10):6439-45.
PMID: 17982032
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation

Kawai T et al. (2006), B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease. Am. J. Pathol., 169(3):987-98.
PMID: 16936272
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Mamoni RL et al. (2006), Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis. Cytokine, 35(3):207-16.
PMID: 17045486
Species: Human

Applications: Binding Assay

Markovic SN et al. (2006), A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood. Clin. Exp. Immunol., 145(3):438-47.
PMID: 16907911
Species: Human
Sample Types: Whole Cells
Applications: ICC

Liao TS et al. (2005), Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone. J. Orthop. Res., 23(1):203-9.
PMID: 15607894
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Ren Y et al. (2005), Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis. Histopathology, 46(6):659-67.
PMID: 15910597
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Ogilvie RL et al. (2005), Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay. J. Immunol., 174(2):953-61.
PMID: 15634918
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Wang P et al. (2004), Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Biochem. J., 380(0):425-33.
PMID: 14992689
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Wolk K et al. (2004), IL-22 increases the innate immunity of tissues. Immunity, 21(2):241-54.
PMID: 15308104
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Janardhan A et al. (2004), HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis. PLoS Biol., 2(1):E6.
PMID: 14737186
Species: Feline
Sample Types: Whole Cells
Applications: Flow Cytometry

Han MK et al. (2003), NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis. J. Leukoc. Biol., 73(4):525-9.
PMID: 12660227
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Takahashi R et al. (2003), In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression. J. Immunol., 171(11):5769-77.
PMID: 14634085

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Customer Reviews for Human CD3 epsilon Antibody (4)

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Human CD3 epsilon Antibody

Name: Danlee Enzler

Application: IHC

Sample Tested: Spleen tissue

Species: Mouse

Verified Customer | Posted 04/18/2018
Human CD3 epsilon Antibody

Name: Leslie Priddy

Application: IHC

Sample Tested: Tumor cell lyastes

Species: Human

Verified Customer | Posted 04/13/2018
Human CD3 epsilon Antibody

Name: Anonymous

Application: Flow

Sample Tested: Blood mononuclear cells (PBMCs)

Species: Human

Verified Customer | Posted 03/21/2018
Human CD3 epsilon Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Human recombinant antibody

Species: Human

Verified Customer | Posted 10/25/2017

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Showing 1 - 4 of 4 reviews Showing All

Product Documents for Human CD3 epsilon Antibody

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Human CD3 epsilon Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human CD3 epsilon Antibody

Scientific Data Examples for Human CD3 epsilon Antibody

Western Blot

Immunocytochemistry

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD3 epsilon

Citations for Human CD3 epsilon Antibody (55)

Customer Reviews for Human CD3 epsilon Antibody (4)

Human CD3 epsilon Antibody

Human CD3 epsilon Antibody

Human CD3 epsilon Antibody

Human CD3 epsilon Antibody

Product Documents for Human CD3 epsilon Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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