Human CD3 epsilon APC-conjugated Antibody

Name: Human CD3 epsilon APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB100A

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB100A

Citations (5)

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Clone UCHT1 was used by HLDA to establish CD designation

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Species Reactivity:	Human
Immunogen:	Human thymocytes (1)
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Mouse IgG₁ Clone # UCHT1
Specificity:	Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

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Applications for Human CD3 epsilon APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD3 epsilon APC-conjugated Antibody

	Flow Cytometry Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: CD3 epsilon

CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha -beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2).

Item	Value
References	Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23: 101
Entrez Gene IDs	916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names	CD3 epsilon, CD3-epsilon, CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), FLJ18683, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE

Citations for Human CD3 epsilon APC-conjugated Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CD3 epsilon APC-conjugated Antibody include:

Kellie N Smith (2016), Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells MBio, 7(3).
PMID: 27247230
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hagen J et al. (2015), Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays. Cells, 4(1):84-95.
PMID: 25679284
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Prendergast A et al. (2010), HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells. AIDS, 24(4):491-502.
PMID: 20071976
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kirwan M et al. (2009), Exogenous TERC alone can enhance proliferative potential, telomerase activity and telomere length in lymphocytes from dyskeratosis congenita patients. Br. J. Haematol., 144(5):771-81.
PMID: 19036115
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Garcia G et al. (2007), Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis. Allergy, 62(2):170-7.
PMID: 17298426

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Showing 1 - 5 of 5 citations Showing All

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Human CD3 epsilon APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human CD3 epsilon APC-conjugated Antibody

Scientific Data Examples for Human CD3 epsilon APC-conjugated Antibody

Flow Cytometry