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Human CD19 PE‑conjugated Antibody
Catalog # FAB4867P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 4G7-2E3
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Product Summary for Human CD19 PE‑conjugated Antibody
Immunogen
Human CLL cells
Specificity
Detects human CD19.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD19 PE‑conjugated Antibody
Detection of CD19 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD19 PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using FAB4867P in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD19
CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.
Product Documents for Human CD19 PE‑conjugated Antibody
Product Specific Notices for Human CD19 PE‑conjugated Antibody
For research use only
Citations for Human CD19 PE‑conjugated Antibody (2)
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Citations are publications that use Bio-Techne products. Selected citations for Human CD19 PE‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Blood
Applications: Flow CytometryI Peluso et al. (2018-10-08), Effects of High Consumption of Vegetables on Clinical, Immunological, and Antioxidant Markers in Subjects at Risk of Cardiovascular Diseases Oxid Med Cell Longev, 2018-12018(0):5417165.
PMID: 30402206 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryH Fayyad-Kaz et al. (2017-01-13), Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming J Transl Med, 2017-015(1):10.
PMID: 28086811
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Customer Reviews for Human CD19 PE‑conjugated Antibody (1)
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Application: Flow CytometrySample Tested: B cell enriched peripheral blood mononuclear cells (PBMC)Species: HumanVerified Customer | Posted 04/10/2018
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