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Human CD3 epsilon Fluorescein-conjugated Antibody

Bio-Techne includes R&D Systems | Catalog # FAB100F

Clone UCHT1 was used by HLDA to establish CD designation

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # UCHT1

Product Summary for Human CD3 epsilon Fluorescein-conjugated Antibody

Immunogen

Human thymocytes (1)

Specificity

Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD3 epsilon Fluorescein-conjugated Antibody

Detection of CD3e antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) or (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD3 epsilon Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 8 publications using FAB100F in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References

  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for Human CD3 epsilon Fluorescein-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD3 epsilon Fluorescein-conjugated Antibody

For research use only

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