Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB100MFV450
Clone UCHT1 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # UCHT1
Product Specifications
Immunogen
Human thymocytes (1)
Specificity
Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody
Detection of CD3 epsilon in PBMC lymphocytes by Flow Cytometry.
PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3 epsilon mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB100MFV450 or (B) unstained. View our protocol for Staining Membrane-associated Proteins.Applications for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody
Application
Recommended Usage
Flow Cytometry
5 µL/106 cells
Sample: PBMC lymphocytes
Sample: PBMC lymphocytes
Spectra Viewer
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD3 epsilon
References
- Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
- Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23:101.
- McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York.
- Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
- Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York.
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody
Product Specific Notices for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody
mFluorTM is a trademark of AAT Bioquest, Inc.
For research use only
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