Can Catalog # CCM038-GMP (GMP Human T cell Media) be used with frozen T cells or frozen PBMCs?

Name: GMP Human T Cell Media
Brand: R&D Systems
SKU: CCM038-GMP-1L

Yes. We have tested CCM038-GMP with both frozen T cells and frozen PBMCs as the starting population. Protocols on the product insert should be followed for each population.

GMP Human T Cell Media

R&D Systems, part of Bio-Techne | Catalog # CCM038-GMP-1L

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Product Datasheet / COA / SDS

For robust T cell expansion with GMP-grade media in xeno-free conditions

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Product Documents
Specific Notices

Key Product Details

Features

GMP Media for the expansion and culture of human T cells under xeno-free conditions.

Key Benefits

Phenol Red-Free formulation in 1 L bottles (CCM038-GMP-1L) or 1 L bags (CCM038-GMP-1B)
Robust T cell expansion under xeno-free conditions
Optimized for use with R&D Systems cytokines and G-Rex® bioreactors
Compatible with a variety of T cell activation methods
Supports T cell expansion from PBMCs

Species

Human

Product Summary for GMP Human T Cell Media

GMP Human T Cell Media is formulated for the ex vivo culture of human T lymphocytes for preclinical or clinical ex vivo use. This media is optimized for T cell expansion in a G-Rex bioreactor with R&D Systems GMP cytokines, in a variety of combinations (IL-7 and IL-15, or alternatively, IL-2). It is also compatible with different cell activation methods, including particle, bead-based, or plate-bound. It is highly recommended to supplement this product with human AB serum. The activation reagents, culture vessel, and cytokine growth factor combinations used with this media should be optimized by application or experimental protocol.

The bagged format of GMP T cell media (CCM038-GMP-1B) is designed to integrate seamlessly into a closed system workflow. Use with ProPak GMP cytokines and a G-Rex bioreactor for a derisked T cell manufacturing process.

Product Specifications

Osmolality

280-330

Sterility Testing

Tested following USP <71> sterility guidelines.

Scientific Data Images for GMP Human T Cell Media

T Cells Maintain High Viability and Undergo Robust Expansion in CCM038-GMP-1L Supplemented With IL-7 & IL-15.

T cells cultured in GMP Human T Cell Media are viable and undergo robust expansion. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 10 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP), and 5% GMP Human AB Serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for viability (A) and cell density (B). Data is the average of 5 T cell donors with error bars ± SD.

CCM038-GMP-1L Supports Robust Transduction Efficiency With IL-7 & IL-15.

GMP Human T Cell Media supports T cell editing with Lentivirus. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 10 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP), and 5% GMP Human AB Serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for expression of the inserted cargo CD19-CAR by staining with CD19 recombinant Fluorokine (Catalog # ATM9269) by flow cytometry (A). Total viable edited cells were calculated by multiplying total live cells by % of CD19-CAR+ (B). Data is the average of 5 T cell donors with error bars ± SD.

CCM038-GMP-1L Supports Generation of T_SCM and T_CM Cells With Low Exhaustion Profiles With IL-7 & IL-15.

GMP Human T Cell Media supports generation of T_SCM and T_CM cells with low PD-1 expression. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 10 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP), and 5% GMP Human AB Serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for CD4+ (A) and CD8+ memory cell subsets (B) and expression of exhaustion marker PD-1 (C). T cell memory subsets are gated on CD45RA and CCR7, as stem cell memory (T_SCM ,CD45RA+ CCR7+), central memory (T_CM, CD45RA- CCR7+), effector memory (T_EM, CD45RA- CCR7-), and effector (T_EFF, CD45RA+ CCR7-). Data is the average of 5 T cell donors with error bars ± SD.

T Cells Maintain High Viability and Undergo Robust Expansion in CCM038-GMP-1L Supplemented With IL-2.

T cells cultured in GMP Human T Cell Media are viable and undergo robust expansion. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-2 GMP Protein (Catalog # BT-002-GMP) and 5% GMP Human AB Serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for viability (A) and cell density (B). Data is the average of 3 T cell donors with error bars ± SD.

CCM038-GMP-1L Supports Robust Transduction Efficiency With IL-2.

GMP Human T Cell Media supports T cell editing with Lentivirus. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-2 GMP Protein (Catalog # BT-002-GMP) and 5% GMP Human AB Serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for expression of the inserted cargo CD19-CAR by staining with CD19 recombinant Fluorokine (Catalog # ATM9269) by flow cytometry (A). Total viable edited cells were calculated by multiplying total live cells by % of CD19-CAR+ (B). Data is the average of 3 T cell donors with error bars ± SD.

CCM038-GMP-1L Supports Generation of T_SCM and T_CM Cells With Low Exhaustion Profiles With IL-2.

GMP Human T Cell Media supports generation of T_SCM and T_CM cells with low PD-1 expression. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-2 GMP Protein (Catalog # BT-002-GMP) and 5% GMP Human AB Serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for CD4+ (A) and CD8+ memory cell subsets (B) and expression of exhaustion marker PD-1 (C). T cell memory subsets are gated on CD45RA and CCR7, as stem cell memory (T_SCM ,CD45RA+ CCR7+), central memory (T_CM, CD45RA- CCR7+), effector memory (T_EM, CD45RA- CCR7-), and effector (T_EFF, CD45RA+ CCR7-). Data is the average of 3 T cell donors with error bars ± SD.

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks.

Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cell density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks.

Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks.

Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cells density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks.

Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 T cell donors with error bars ± SD.

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Kit Contents for GMP Human T Cell Media

1 L Bottle of GMP Human T Cell Media (CCM038-GMP-1L), or
1 L Bag of GMP Human T Cell Media (CCM038-GMP-1B)

Formulation, Preparation, and Storage

Shipping

The product is shipped at 2-8 °C. Upon receipt, store immediately at the recommended temperature. Protect from light.

Storage

Store the unopened product at 2-8 °C and protected from light. Do not use past the use by date on the Certificate of Analysis.

Product Documents for GMP Human T Cell Media

Product Datasheets

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COA

SDS

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Product Specific Notices for GMP Human T Cell Media

For preclinical, or clinical ex vivo use
This reagent should not be used beyond the use by date indicated on the CoA
Results may vary due to variations among primary T lymphocyte populations derived from different donors

Citations
Reviews

GMP Human T Cell Media