GMP Human AB Serum

GMP Human AB Serum supports high expansion and viability in T cell cultures Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) OR IL-2 (BT-002-GMP) cytokines. Using fresh complete media, T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), fold expansion (B), and cell density (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype when cultured with GMP Human AB Serum Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) OR IL-2 (BT-002-GMP) cytokines. Using fresh complete media, T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell memory phenotype was analyzed for either the CD4+ (A) or CD8+ (B) T cell population after 7 days in culture. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 purified T cell donors with error bars ± SD.

Transduction of T cells cultured with GMP Human AB Serum T cells from 3 different donors were cultured in Bio-Techne’s CCM038 media supplemented with 10 ng/mL IL-7 and IL-15 and 5% GMP Human AB Serum. Cells were thawed, plated in a 6M G-rex and transduced one day post-thaw via lentivirus (EF1a-CD19-DHFReGFP, MOI of 5). The percentage of GFP and CD19 CAR expressed in T cells is shown after 8 total days in culture. Transduction efficiency is shown as a combination of 3 donors (A). A luciferase-base kill assay was performed to measure T cell killing of Nalm-6 cancer cells. T cells cultured in A were cryopreserved prior to kill assay. T cells were thawed and plated with Nalm-6 cells at effector: target ratios ranging from 5:1 to 0.08:1 and cultured for 24 hours. For transduced T cells, cells were plated based on CD19 CAR+ positive cells determined via flow cytometry prior to freezing. Data (B) shows the three T cell donors grown with GMP Human AB Serum and includes transduced samples (TD) and grow out samples (No TD). Transduced T cells grown in media containing GMP Human AB Serum showed high percentage killing of Nalm-6 cancer cells.

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cell density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cells density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 T cell donors with error bars ± SD.