Simple Plex Human IFN-gamma (3rd Gen) Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002574
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IFN-gamma (3rd Gen) Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IFN-gamma assay.
Inter-Assay Precision (Precision between assays) Replicates of each IFN-gamma control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IFN-gamma.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 38 | 39 |
| Mean (pg/mL) | 21.1 | 1175 | 23.4 | 1192 |
| Standard Deviation | 1.48 | 33.4 | 2.52 | 99.4 |
| CV% | 7.0 | 2.8 | 10.8 | 8.3 |
Recovery for Simple Plex Human IFN-gamma (3rd Gen) Cartridge
Recovery for the human IFN-gamma assays was evaluated at three different spiked concentrations of IFN-gamma protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cerebrospinal Fluid (n=6) | 94 | 91-98 |
| EDTA Plasma (n=4) | 89 | 88-90 |
| Heparin Plasma (n=4) | 90 | 83-99 |
| Serum (n=4) | 95 | 83-102 |
Linearity
Samples containing and/or spiked with high concentrations of human IFN-gamma were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IFN-gamma (3rd Gen) Cartridge
Simple Plex Human IFN-gamma Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IFN-gamma Assay. Assay range is 0.17-4000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IFN-gamma Products
Product Documents for Simple Plex Human IFN-gamma (3rd Gen) Cartridge
Product Specific Notices for Simple Plex Human IFN-gamma (3rd Gen) Cartridge
For research use only