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Proteome Profiler Mouse XL Cytokine Array
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Lysate. Lysates from Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (200 µg lysate, 10 minute exposure). |
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Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate. Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (200 µg lysate, 10 minute exposure). |
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Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate. Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (500 µL of cell culture supernate, 5 minute exposure). |
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, relative expression levels of mouse cytokines and chemokines in samples can be determined using the following procedure:
- Prepare membrane and incubate with prepared sample
- Incubate the membrane with Detection Antibody Cocktail
- Incubate the membrane array with Streptavidin-HRP
- Develop the membrane array with Chemi Reagents 1 and 2
- Expose the membrane array to autoradiography film
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
Other Supplies Required
Reagents
- Aprotinin
- Leupeptin (Catalog #EI002)
- Pepstatin (Catalog #EI003)
- Igepal® CA-630
- Deionized or distilled water
Materials
- Pipettes and pipette tips
- Gloves
- Plastic container with the capacity to hold 50 mL (for washing the arrays)
- Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
- Plastic wrap
- Absorbent lab wipes (KimWipes® or equivalent)
- Paper towels
- X-ray film (Kodak BioMax™ Light-1) or equivalent
- Flat-tipped tweezers
Equipment
- Rocking platform shaker
- Microcentrifuge
- Autoradiography cassette
- Film developer
- Flatbed scanner with transparency adapter capable of transmission mode
- Computer capable of running image analysis software and Microsoft Excel
Other Supplies Required for Cell Lysate Samples
- Phosphate-Buffered Saline (PBS)
- Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
Other Supplies Required for Tissue Lysate Samples
- PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
- Triton™ X-100
Procedure Overview
R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028)
Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.
Place each array membrane in a separate well of the 4-Well Multi-dish.
Incubate for one hour on a rocking platform shaker.
Add 0.5 mL Array Buffer 4 to each sample.
Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.
Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.
Incubate overnight at 2 °C to 8 °C on a rocking platform.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.
Add 30 µL of Detection Antibody Cocktail to 1.5 mL of Array Buffer 4/6 for each array.
Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.
Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.
Incubate for one hour on a rocking platform shaker.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.
Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.
Place the array membrane in the diluted Streptavidin-HRP solution.
Incubate for 30 minutes on a rocking platform shaker.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Place the array membrane on a plastic sheet protector.
Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.
Cover the membrane with the top sheet of the plastic protector.
Incubate for 1 minute.
Blot off excess Chemi Reagent Mix.
Wrap the membrane and sheet protector in plastic wrap.
Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.
Citations for Proteome Profiler Mouse XL Cytokine Array (97)
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Mouse XL Cytokine Array include:
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Species: Mouse
Sample Types: Tissue Homogenates
HJ Lee et al. (2022), Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke Neural regeneration research, 17(7):1556-1565.
PMID: 34916441 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Meng et al. (2022), Tumor-derived Jagged1 promotes cancer progression through immune evasion Cell Reports, 38(10):110492.
PMID: 35263601 -
Species: Mouse
Sample Types: Tissue Homogenates
FX Yu et al. (2022), The impact of sensory neuropathy and inflammation on epithelial wound healing in diabetic corneas Progress in retinal and eye research, 0(0):101039.
PMID: 34991965 -
Species: Mouse
Sample Types: Whole Cell
TN Pham et al. (2022), Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity JCI Insight, 0(0).
PMID: 35380995 -
Species: Mouse
Sample Types: Cell Culture Supernants
L Zhong et al. (2022), Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation JCI Insight, 7(7).
PMID: 35393948 -
Species: Mouse
Sample Types: Cell Culture Supernates
S Ryu et al. (2022), Loss of RNA binding protein HuD facilitates the production of the senescence-associated secretory phenotype Cell Death Disease, 2022;13(4):329.
PMID: 35411051 -
Species: Mouse
Sample Types: Plasma
K Sundaram et al. (2022), Garlic exosome-like nanoparticles reverse high-fat diet induced obesity via the gut/brain axis Theranostics, 12(3):1220-1246.
PMID: 35154484 -
Species: Mouse
Sample Types: Plasma
A Kumar et al. (2022), Ginger nanoparticles mediated induction of Foxa2 prevents high-fat diet-induced insulin resistance Theranostics, 12(3):1388-1403.
PMID: 35154496 -
Species: Mouse
Sample Types: Cell Lysates
BG Hunt et al. (2022), RON (MST1R) and HGFL (MST1) Co-Overexpression Supports Breast Tumorigenesis through Autocrine and Paracrine Cellular Crosstalk Cancers, 14(10).
PMID: 35626096 -
Species: Mouse
Sample Types: Protein Lysates
HC Pan et al. (2022), Essential Roles of the Histone Demethylase KDM4C in Renal Development and Acute Kidney Injury International Journal of Molecular Sciences, 23(16).
PMID: 36012577 -
Species: Mouse
Sample Types: Tissue Lysates
HY Kim et al. (2022), Recombinant FGF21 Attenuates Polychlorinated Biphenyl-Induced NAFLD/NASH by Modulating Hepatic Lipocalin-2 Expression International Journal of Molecular Sciences, 23(16).
PMID: 36012166 -
VC Sanchez et al. (2022), Host CLIC4 expression in the tumor microenvironment is essential for breast cancer metastatic competence PloS Genetics, 18(6):e1010271.
PMID: 35727842 -
Species: Mouse
Sample Types: Tissue Homogenates
S Räuber et al. (2022), Modulation of Rxralpha Expression in Mononuclear Phagocytes Impacts on Cardiac Remodeling after Ischemia-Reperfusion Injury Biomedicines, 10(6).
PMID: 35740296 -
Species: Mouse
Sample Types: Tissue Homogenates
N Tisch et al. (2022), Caspase-8 in endothelial cells maintains gut homeostasis and prevents small bowel inflammation in mice Embo Molecular Medicine, 0(0):e14121.
PMID: 35491615 -
Species: Mouse
Sample Types: Cell Culture Supernates
HC West et al. (2022), Loss of T�cell tolerance in the skin following immunopathology is linked to failed restoration of the dermal niche by recruited macrophages Cell Reports, 39(7):110819.
PMID: 35584681 -
Species: Mouse
Sample Types: Serum
M Balogh et al. (2022), Sensory neuron dysfunction in orthotopic mouse models of colon cancer Journal of Neuroinflammation, 19(1):204.
PMID: 35962398 -
Species: Mouse
Sample Types: Serum
JY Hou et al. (2021), Integration of transcriptomics and system pharmacology to reveal the therapeutic mechanism underlying Qingfei Xiaoyan Wan to treat allergic asthma Journal of ethnopharmacology, 278(0):114302.
PMID: 34090911 -
Species: Mouse
Sample Types: Cell Culture Supernates
E Yeini et al. (2021), P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature Communications, 12(1):1912.
PMID: 33771989 -
Species: Mouse
Sample Types: Peritoneal Fluid
T Ito et al. (2021), Cell barrier function of resident peritoneal macrophages in post-operative adhesions Nature Communications, 12(1):2232.
PMID: 33854051 -
Species: Mouse
Sample Types: Cell Culture Supernates
X Sun et al. (2021), Preventing tumor progression to the bone by induced tumor-suppressing MSCs Theranostics, 11(11):5143-5159.
PMID: 33859739 -
Species: Mouse
Sample Types: Cell Lysates
K Li et al. (2021), CD8+ T cell immunity blocks the metastasis of carcinogen-exposed breast cancer Science Advances, 7(25).
PMID: 34144976 -
Species: Mouse
Sample Types: Tissue Homogenates
D Paramos-de et al. (2021), Targeting senescent cells improves functional recovery after spinal cord injury Cell Reports, 36(1):109334.
PMID: 34233184 -
Species: Mouse
Sample Types: Tissue Homogenates
MJ Strowitzki et al. (2021), Inhibition of HIF-prolyl hydroxylases improves healing of intestinal anastomoses JCI Insight, 0(0).
PMID: 33784253 -
Species: Mouse
Sample Types: Plasma
R Pandey et al. (2021), JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific Reports, 11(1):7372.
PMID: 33795715 -
Species: Mouse
Sample Types: Tissue Homogenates
Q Li et al. (2021), Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36 Brain, Behavior, and Immunity, 94(0):437-457.
PMID: 33588074 -
Species: Mouse
Sample Types: Tissue Homogenates
CS Thomson et al. (2021), WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth Oncogene, 40(24):4229-4241.
PMID: 34079083 -
Species: Mouse
Sample Types: Serum
T Masaki et al. (2021), GIP_HUMAN[22-51] is a new proatherogenic peptide identified by native plasma peptidomics Scientific Reports, 11(1):14470.
PMID: 34262109 -
Species: Mouse
Sample Types: Cell Culture Supernates
LM Snyder et al. (2021), Induction of IL-12p40 and type 1 immunity by Toxoplasma gondii in the absence of the TLR-MyD88 signaling cascade PloS Pathogens, 17(10):e1009970.
PMID: 34597344 -
Species: Mouse
Sample Types: Cell Culture Supernates
D Chanda et al. (2021), Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells Elife, 10(0).
PMID: 34528872 -
Species: Mouse
Sample Types: Plasma
R Samain et al. (2021), Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 33482394 -
Species: Mouse
Sample Types: Serum
Y Feng et al. (2021), Mechanical Loading-Driven Tumor Suppression Is Mediated by Lrp5-Dependent and Independent Mechanisms Cancers, 13(2).
PMID: 33450808 -
Species: Mouse
Sample Types: Cell Culture Supernates
N Ogawa et al. (2021), Trimebutine suppresses Toll-like receptor 2/4/7/8/9 signaling pathways in macrophages Archives of biochemistry and biophysics, 711(0):109029.
PMID: 34517011 -
Species: Mouse
Sample Types: Plasma
F Yao et al. (2021), A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 12(1):7333.
PMID: 34921145 -
Species: Mouse
Sample Types: Cell Culture Supernates
HL Desu et al. (2021), TNFR2 Signaling Regulates the Immunomodulatory Function of Oligodendrocyte Precursor Cells Cells, 10(7).
PMID: 34359956 -
Species: Mouse
Sample Types: Serum
P Mamidi et al. (2021), MK2a inhibitor CMPD1 abrogates chikungunya virus infection by modulating actin remodeling pathway Oncogene, 17(11):e1009667.
PMID: 34780576 -
Species: Mouse
Sample Types: Plasma
A Kumar et al. (2021), High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Nature Communications, 12(1):213.
PMID: 33431899 -
Species: Mouse
Sample Types: Tissue Homogenates
JD Bryant et al. (2021), Neuroimmune mechanisms of cognitive impairment in a mouse model of Gulf War illness Brain, Behavior, and Immunity, 0(0).
PMID: 34333111 -
Species: Mouse
Sample Types: Cell Culture Supernates
S Xiao et al. (2021), Innate immune regulates cutaneous sensory IL-13 receptor alpha 2 to promote atopic dermatitis Brain, Behavior, and Immunity, 98(0):28-39.
PMID: 34391816 -
Species: Mouse
Sample Types: Cell Culture Supernates
M Mizuno et al. (2021), Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell Interaction Cancers, 13(16).
PMID: 34439392 -
Species: Mouse
Sample Types: Protein Lysates
P Jiranugrom et al. (2020), NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet Genes (Basel), 11(5).
PMID: 32438638 -
Species: Mouse
Sample Types: Cell Culture Supernates
HL Mercer et al. (2020), Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity PLoS Pathog., 16(5):e1008572.
PMID: 32413093 -
Species: Mouse
Sample Types: Serum
Y Cheng et al. (2020), Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages PLoS Pathog., 16(5):e1008569.
PMID: 32463840 -
Species: Mouse
Sample Types: Serum
D Ren et al. (2020), Sestrin2 modulates cardiac inflammatory response through maintaining redox homeostasis during ischemia and reperfusion Redox Biol, 34(0):101556.
PMID: 32447260 -
Species: Mouse
Sample Types: Cell Lysates
AM Decker et al. (2020), Adrenergic Blockade Promotes Maintenance of Dormancy in Prostate Cancer Through Upregulation of GAS6 Transl Oncol, 13(7):100781.
PMID: 32361123 -
Species: Mouse
Sample Types: Cell Culture Supernates
AA Hartman et al. (2020), YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells Stem Cell Reports, 14(4):730-743.
PMID: 32243844 -
Species: Mouse
Sample Types: Tissue Homogenates
W He et al. (2020), High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation Nat Commun, 11(1):1732.
PMID: 32265505 -
Species: Mouse
Sample Types: Tissue Homogenates
HY Lin et al. (2020), SUMO E3 ligase PIAS1 is a potential biomarker indicating stress susceptibility Psychoneuroendocrinology, 120(0):104800.
PMID: 32688147 -
Species: Mouse
Sample Types: Tissue Homogenates
Y Liu et al. (2020), Triterpenoids Extracted From Antrodia cinnamomea Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice via Suppression Inflammatory Response Front Microbiol, 11(0):1113.
PMID: 32719658 -
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
K Hirashima et al. (2020), The mechanism of Tyk2 deficiency-induced immunosuppression in mice involves robust IL-10 production in macrophages Cytokine, 130(0):155077.
PMID: 32208335 -
Species: Mouse
Sample Types: Cell Culture Lysates
F Demirciogl et al. (2020), Cancer associated fibroblast FAK regulates malignant cell metabolism Nat Commun, 11(1):1290.
PMID: 32157087 -
Species: Mouse
Sample Types: Tissue Lysates
X Zhou et al. (2020), Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2 Nat. Neurosci., 23(3):337-350.
PMID: 32112058 -
Species: Mouse
Sample Types: Whole Cells
CC Hu et al. (2020), Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects Int J Mol Sci, 21(15).
PMID: 32756396 -
Species: Mouse
Sample Types: Tissue Homogenates
MT Hoffman et al. (2020), The Gustatory Sensory G-Protein GNAT3 Suppresses Pancreatic Cancer Progression in Mice Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32882403 -
Species: Mouse
Sample Types: Serum
MB Preda et al. (2020), Evidence of mesenchymal stromal cell adaptation to local microenvironment following subcutaneous transplantation J. Cell. Mol. Med., 0(0).
PMID: 32785979 -
Species: Mouse
Sample Types: Plasma
S Marquard et al. (2020), Yes-associated protein (YAP) induces a secretome phenotype and transcriptionally regulates plasminogen activator Inhibitor-1 (PAI-1) expression in hepatocarcinogenesis Cell Commun Signal, 18(1):166.
PMID: 33097058 -
Species: Mouse
Sample Types: Tissue Homogenates
YJ Park et al. (2020), Bisphenol A exposure increases epididymal susceptibility to infection in mice Ecotoxicol Environ Saf, 208(0):111476.
PMID: 33091778 -
Species: Mouse
Sample Types: Serum
HJ Wang et al. (2020), Adaptive thermogenesis enhances the life-threatening response to heat in mice with an Ryr1 mutation Nat Commun, 11(1):5099.
PMID: 33037202 -
Species: Mouse
Sample Types: Cell Lysates
JS Russell et al. (2020), Dietary &alpha-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection Nutrients, 12(9).
PMID: 32887376 -
Species: Mouse
Sample Types: Plasma
WN Jin et al. (2020), Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition Nature Neuroscience, 0(0).
PMID: 33257875 -
Species: Mouse
Sample Types: Cell Culture Supernates
A Banisadr et al. (2020), EGFRvIII uses intrinsic and extrinsic mechanisms to reduce glioma adhesion and increase migration J Cell Sci, 0(0).
PMID: 33243756 -
Species: Mouse
Sample Types: Cell Culture Supernates
K Lim et al. (2020), In situ neutrophil efferocytosis shapes T cell immunity to influenza infection Nat. Immunol., 0(0).
PMID: 32747818 -
Species: Mouse
Sample Types: Cell Culture Supernates
Y Guan et al. (2020), Senescence-activated enhancer landscape orchestrates the senescence-associated secretory phenotype in murine fibroblasts Nucleic Acids Res, 0(0).
PMID: 33045748 -
Species: Mouse
Sample Types: Serum
R Fernandez- et al. (2020), Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells Nat Commun, 11(1):5982.
PMID: 33239617 -
Species: Mouse
Sample Types: Cell Lysates
B Lee et al. (2020), An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1 Autophagy, 0(0):1-18.
PMID: 32579471 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Huang et al. (2020), Hyperactivity of Innate Immunity Triggers Pain via TLR2-IL-33-Mediated Neuroimmune Crosstalk Cell Rep, 33(1):108233.
PMID: 33027646 -
Species: Mouse
Sample Types: Cell Culture Supernates
CC Hu et al. (2020), Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects Int J Mol Sci, 21(15).
PMID: 32756396 -
Species: Mouse
Sample Types: Serum
MB Preda et al. (2020), Evidence of mesenchymal stromal cell adaptation to local microenvironment following subcutaneous transplantation J. Cell. Mol. Med., 0(0).
PMID: 32785979 -
Species: Mouse
Sample Types: Tissue Homogenates
K Lim et al. (2020), In situ neutrophil efferocytosis shapes T cell immunity to influenza infection Nat. Immunol., 0(0).
PMID: 32747818 -
Species: Mouse
Sample Types: Plasma
J Liu et al. (2019), Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output EBioMedicine, 0(0).
PMID: 30827929 -
Species: Mouse
Sample Types: Cell Lysates
W Wang et al. (2019), Prostate cancer promotes a vicious cycle of bone metastasis progression through inducing osteocytes to secrete GDF15 that stimulates prostate cancer growth and invasion Oncogene, 0(0).
PMID: 30755731 -
Species: Mouse
Sample Types: Tissue Homogenates
M Malehmir et al. (2019), Platelet GPIb? is a mediator and potential interventional target for NASH and subsequent liver cancer Nat. Med., 25(4):641-655.
PMID: 30936549 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Schyns et al. (2019), Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung Nat Commun, 10(1):3964.
PMID: 31481690 -
Species: Mouse
Sample Types: Matrix Bound Nanovesicles
GS Hussey et al. (2019), Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway J Immunol Regen Med, 3(0):26-35.
PMID: 31656879 -
Species: Mouse
Sample Types: Tissue Homogenates
F Hempel et al. (2019), Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice Cells, 8(10).
PMID: 31591328 -
Species: Mouse
Sample Types: Cell Culture Supernates
A El-Kenawi et al. (2019), Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer Br. J. Cancer, 121(7):556-566.
PMID: 31417189 -
Species: Mouse
Sample Types: Whole Cells
K Haruwaka et al. (2019), Dual microglia effects on blood brain barrier permeability induced by systemic inflammation Nat Commun, 10(1):5816.
PMID: 31862977 -
Species: Mouse
Sample Types: Cell Culture Supernates
A Gordon-Wee et al. (2019), Tumour-Derived Laminin alpha5 (LAMA5) Promotes Colorectal Liver Metastasis Growth, Branching Angiogenesis and Notch Pathway Inhibition Cancers (Basel), 11(5).
PMID: 31064120 -
Species: Mouse
Sample Types: Serum
P Chang et al. (2018), Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine Cell Death Dis, 9(2):154.
PMID: 29402989 -
Species: Mouse
Sample Types: Tissue Homogenates
A Calcinotto et al. (2018), IL-23 secreted by myeloid cells drives castration-resistant prostate cancer Nature, 0(0).
PMID: 29950727 -
Species: Mouse
Sample Types: Cell Culture Supernates
KB Gama et al. (2018), Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation Stem Cells Int, 2018(0):8179013.
PMID: 29535781 -
Species: Mouse
Sample Types: Tissue Homogenates
RA Brady et al. (2018), Comparison of the immune response during acute and chronic Staphylococcus aureus infection PLoS ONE, 13(3):e0195342.
PMID: 29596507 -
Species: Mouse
Sample Types: Cell Culture Supernates
X Sun et al. (2018), Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulatingadipose stromal interleukin 6 production in mice J. Biol. Chem., 0(0).
PMID: 29298894 -
Species: Mouse
Sample Types: Cell Lysates
HR Kim et al. (2018), T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells Nat Commun, 9(1):3630.
PMID: 30194420 -
Species: Mouse
Sample Types: Plasma
T Szatmári et al. (2018), Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood Int. J. Radiat. Biol., 0(0):1-31.
PMID: 29533121 -
Species: Mouse
Sample Types: Cell Lysates
T Kobori et al. (2018), Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis Front Immunol, 9(0):334.
PMID: 29559970 -
Species: Mouse
Sample Types: Peritoneal Fluid
MJ Strowitzki et al. (2017), Pharmacological HIF-inhibition attenuates postoperative adhesion formation Sci Rep, 7(1):13151.
PMID: 29030625 -
Species: Mouse
Sample Types: Tissue Homogenates
WN Jin et al. (2017), Depletion of microglia exacerbates postischemic inflammation and brain injury J. Cereb. Blood Flow Metab., 0(0):271678X176941.
PMID: 28273719 -
Species: Mouse
Sample Types: Cell Culture Supernates
C Carmona-Fo et al. (2017), Metabolic origins of spatial organization in the tumor microenvironment Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28246332 -
Species: Mouse
Sample Types: Cell Culture Supernates
BV Howley et al. (2017), A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Oncogene, 0(0).
PMID: 29249802 -
Species: Mouse
Sample Types: Cell Culture Supernates
D Ribet et al. (2017), Promyelocytic Leukemia Protein (PML) Controls Listeria monocytogenes Infection MBio, 8(1).
PMID: 28074026 -
Species: Mouse
Sample Types: Cell Lysates
K Murakami et al. (2017), A Jak1/2 inhibitor, baricitinib, inhibits osteoclastogenesis by suppressing RANKL expression in osteoblasts in vitro PLoS ONE, 12(7):e0181126.
PMID: 28708884 -
Species: Mouse
Sample Types: Tissue Homogenates
Á Sánchez et al. (2017), Map3k8 controls granulocyte colony-stimulating factor production and neutrophil precursor proliferation in lipopolysaccharide-induced emergency granulopoiesis Sci Rep, 7(1):5010.
PMID: 28694430 -
Species: Mouse
Sample Types: Tissue Homogenates
X Lu et al. (2017), Effective combinatorial immunotherapy for castration-resistant prostate cancer Nature, 543(7647):728-732.
PMID: 28321130 -
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
Nan Chiang (2016), Novel Resolvin D2 Receptor Axis in Infectious Inflammation J. Immunol, 0(0).
PMID: 27994074 -
Species: Mouse
Sample Types: Cell Culture Supernates
Qi Liu (2016), Evaluation of PLGA containing anti-CTLA4 inhibited endometriosis progression by regulating CD4+CD25+Treg cells in peritoneal fluid of mouse endometriosis model Eur J Pharm Sci, 0(0).
PMID: 27989857 -
Species: Mouse
Sample Types: Cell Lysates
(2016), Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 27994144 -
Species: Mouse
Sample Types: Serum
Fernandez-Ruiz R et al. (0), Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells. Nat Commu11(1):5982.
PMID: 33239617
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Customer Reviews for Proteome Profiler Mouse XL Cytokine Array (29)
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Verified Customer | Posted 10/16/2022Conditioned medium of macrophages (36h) isolated from metastatic lungs.
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Verified Customer | Posted 08/28/2022This kit is really great!
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Verified Customer | Posted 08/07/2022Secretome of 4T1 murine breast cancer cells.
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Verified Customer | Posted 08/07/2022Cytokine array of the secretome of MLE12 cells.
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Verified Customer | Posted 07/11/2022
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Verified Customer | Posted 12/02/2021
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Verified Customer | Posted 10/21/2021
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Verified Customer | Posted 09/16/2021
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Verified Customer | Posted 08/10/2021We used the array kit for profiling inflammatory cytokines in plasma samples from mice. I trust that it works very well.
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Verified Customer | Posted 03/14/2021
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Verified Customer | Posted 09/17/2020Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Readout is not a fluorescence so you don't need a special fluorescence reader. If you are doing Western blotting, you can do this easily.
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Verified Customer | Posted 02/12/2020Mouse breast tumor tissues
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Verified Customer | Posted 01/25/2020
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Verified Customer | Posted 01/06/2020Excellent array!!
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Verified Customer | Posted 06/06/2019
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Verified Customer | Posted 03/13/2019
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Verified Customer | Posted 12/05/2018
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Verified Customer | Posted 11/11/2018Excellent specificity and detection for large number of cytokines. Works perfectly with little optimization.
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Verified Customer | Posted 11/05/2018Pancreas lysate. 750ug protein/ membrane
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Verified Customer | Posted 07/18/2018
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Verified Customer | Posted 04/01/2018
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Verified Customer | Posted 03/22/2018
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Verified Customer | Posted 12/07/2017200 uL pooled serum from wild-type C57Bl/6 mice.5 min exposure time
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Verified Customer | Posted 08/17/2017
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Verified Customer | Posted 07/17/2017
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Verified Customer | Posted 05/23/2017
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Verified Customer | Posted 11/28/2016150 uL of mouse serum
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Verified Customer | Posted 09/30/2016This is a great kit. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. I strongly recommend it.
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Verified Customer | Posted 08/26/2016The sample was cell culture supernatant collected from MEF.
There are no reviews that match your criteria.
FAQs for Proteome Profiler Mouse XL Cytokine Array
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
