Proteome Profiler Mouse XL Cytokine Array
Order Information
Shipping Information
$35 to United States
Products in stock will be shipped in 1-3 business days unless specified.
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, relative expression levels of mouse cytokines and chemokines in samples can be determined using the following procedure:
- Prepare membrane and incubate with prepared sample
- Incubate the membrane with Detection Antibody Cocktail
- Incubate the membrane array with Streptavidin-HRP
- Develop the membrane array with Chemi Reagents 1 and 2
- Expose the membrane array to autoradiography film
- Rectangular 4-Well Multi-dish
- 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
Reagents
- Aprotinin
- Leupeptin (Catalog #EI002)
- Pepstatin (Catalog #EI003)
- Igepal® CA-630
- Deionized or distilled water
Materials
- Pipettes and pipette tips
- Gloves
- Plastic container with the capacity to hold 50 mL (for washing the arrays)
- Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
- Plastic wrap
- Absorbent lab wipes (KimWipes® or equivalent)
- Paper towels
- X-ray film (Kodak BioMax™ Light-1) or equivalent
- Flat-tipped tweezers
Equipment
- Rocking platform shaker
- Microcentrifuge
- Autoradiography cassette
- Film developer
- Flatbed scanner with transparency adapter capable of transmission mode
- Computer capable of running image analysis software and Microsoft Excel
Other Supplies Required for Cell Lysate Samples
- Phosphate-Buffered Saline (PBS)
- Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
Other Supplies Required for Tissue Lysate Samples
- PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
- Triton™ X-100
R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028)
Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

Place each array membrane in a separate well of the 4-Well Multi-dish.
Incubate for one hour on a rocking platform shaker.

Add 0.5 mL Array Buffer 4 to each sample.
Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.

Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.
Incubate overnight at 2 °C to 8 °C on a rocking platform.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.


Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.

Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.
Incubate for one hour on a rocking platform shaker.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.
Place the array membrane in the diluted Streptavidin-HRP solution.
Incubate for 30 minutes on a rocking platform shaker.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Place the array membrane on a plastic sheet protector.
Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.
Cover the membrane with the top sheet of the plastic protector.
Incubate for 1 minute.
Blot off excess Chemi Reagent Mix.
Wrap the membrane and sheet protector in plastic wrap.
Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.

Citations (40)
Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Mouse XL Cytokine Array include:
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Species: Mouse
Sample Types: Tissue Lysates
X Zhou et al. (2020), Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2 Nat. Neurosci., 23(3):337-350.
PMID: 32112058 -
Species: Mouse
Sample Types: Cell Culture Supernates
AA Hartman et al. (2020), YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells Stem Cell Reports, 14(4):730-743.
PMID: 32243844 -
Species: Mouse
Sample Types: Protein Lysates
P Jiranugrom et al. (2020), NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet Genes (Basel), 11(5):.
PMID: 32438638 -
Species: Mouse
Sample Types: Cell Culture Supernates
HL Mercer et al. (2020), Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity PLoS Pathog., 16(5):e1008572.
PMID: 32413093 -
Species: Mouse
Sample Types: Serum
Y Cheng et al. (2020), Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages PLoS Pathog., 16(5):e1008569.
PMID: 32463840 -
Species: Mouse
Sample Types: Serum
D Ren et al. (2020), Sestrin2 modulates cardiac inflammatory response through maintaining redox homeostasis during ischemia and reperfusion Redox Biol, 34(0):101556.
PMID: 32447260 -
Species: Mouse
Sample Types: Cell Lysates
AM Decker et al. (2020), Adrenergic Blockade Promotes Maintenance of Dormancy in Prostate Cancer Through Upregulation of GAS6 Transl Oncol, 13(7):100781.
PMID: 32361123 -
Species: Mouse
Sample Types: Tissue Homogenates
W He et al. (2020), High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation Nat Commun, 11(1):1732.
PMID: 32265505 -
Species: Mouse
Sample Types: Tissue Homogenates
HY Lin et al. (2020), SUMO E3 ligase PIAS1 is a potential biomarker indicating stress susceptibility Psychoneuroendocrinology, 120(0):104800.
PMID: 32688147 -
Species: Mouse
Sample Types: Tissue Homogenates
Y Liu et al. (2020), Triterpenoids Extracted From Antrodia cinnamomea Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice via Suppression Inflammatory Response Front Microbiol, 11(0):1113.
PMID: 32719658 -
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
K Hirashima et al. (2020), The mechanism of Tyk2 deficiency-induced immunosuppression in mice involves robust IL-10 production in macrophages Cytokine, 130(0):155077.
PMID: 32208335 -
Species: Mouse
Sample Types: Cell Culture Lysates
F Demirciogl et al. (2020), Cancer associated fibroblast FAK regulates malignant cell metabolism Nat Commun, 11(1):1290.
PMID: 32157087 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Schyns et al. (2019), Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung Nat Commun, 10(1):3964.
PMID: 31481690 -
Species: Mouse
Sample Types: Whole Cells
K Haruwaka et al. (2019), Dual microglia effects on blood brain barrier permeability induced by systemic inflammation Nat Commun, 10(1):5816.
PMID: 31862977 -
Species: Mouse
Sample Types: Cell Culture Supernates
A El-Kenawi et al. (2019), Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer Br. J. Cancer, 121(7):556-566.
PMID: 31417189 -
Species: Mouse
Sample Types: Tissue Homogenates
F Hempel et al. (2019), Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice Cells, 8(10):.
PMID: 31591328 -
Species: Mouse
Sample Types: Matrix Bound Nanovesicles
GS Hussey et al. (2019), Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway J Immunol Regen Med, 3(0):26-35.
PMID: 31656879 -
Species: Mouse
Sample Types: Tissue Homogenates
M Malehmir et al. (2019), Platelet GPIb? is a mediator and potential interventional target for NASH and subsequent liver cancer Nat. Med., 25(4):641-655.
PMID: 30936549 -
Species: Mouse
Sample Types: Cell Lysates
W Wang et al. (2019), Prostate cancer promotes a vicious cycle of bone metastasis progression through inducing osteocytes to secrete GDF15 that stimulates prostate cancer growth and invasion Oncogene, 0(0):.
PMID: 30755731 -
Species: Mouse
Sample Types: Plasma
J Liu et al. (2019), Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output EBioMedicine, 0(0):.
PMID: 30827929 -
Species: Mouse
Sample Types: Plasma
T Szatmári et al. (2018), Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood Int. J. Radiat. Biol., 0(0):1-31.
PMID: 29533121 -
Species: Mouse
Sample Types: Cell Culture Supernates
KB Gama et al. (2018), Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation Stem Cells Int, 2018(0):8179013.
PMID: 29535781 -
Species: Mouse
Sample Types: Tissue Homogenates
RA Brady et al. (2018), Comparison of the immune response during acute and chronic Staphylococcus aureus infection PLoS ONE, 13(3):e0195342.
PMID: 29596507 -
Species: Mouse
Sample Types: Cell Culture Supernates
X Sun et al. (2018), Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulatingadipose stromal interleukin 6 production in mice J. Biol. Chem., 0(0):.
PMID: 29298894 -
Species: Mouse
Sample Types: Serum
P Chang et al. (2018), Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine Cell Death Dis, 9(2):154.
PMID: 29402989 -
Species: Mouse
Sample Types: Cell Lysates
T Kobori et al. (2018), Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis Front Immunol, 9(0):334.
PMID: 29559970 -
Species: Mouse
Sample Types: Cell Lysates
HR Kim et al. (2018), T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells Nat Commun, 9(1):3630.
PMID: 30194420 -
Species: Mouse
Sample Types: Tissue Homogenates
A Calcinotto et al. (2018), IL-23 secreted by myeloid cells drives castration-resistant prostate cancer Nature, 0(0):.
PMID: 29950727 -
Species: Mouse
Sample Types: Tissue Homogenates
Á Sánchez et al. (2017), Map3k8 controls granulocyte colony-stimulating factor production and neutrophil precursor proliferation in lipopolysaccharide-induced emergency granulopoiesis Sci Rep, 7(1):5010.
PMID: 28694430 -
Species: Mouse
Sample Types: Cell Lysates
K Murakami et al. (2017), A Jak1/2 inhibitor, baricitinib, inhibits osteoclastogenesis by suppressing RANKL expression in osteoblasts in vitro PLoS ONE, 12(7):e0181126.
PMID: 28708884 -
Species: Mouse
Sample Types: Cell Culture Supernates
D Ribet et al. (2017), Promyelocytic Leukemia Protein (PML) Controls Listeria monocytogenes Infection MBio, 8(1):.
PMID: 28074026 -
Species: Mouse
Sample Types: Tissue Homogenates
X Lu et al. (2017), Effective combinatorial immunotherapy for castration-resistant prostate cancer Nature, 543(7647):728-732.
PMID: 28321130 -
Species: Mouse
Sample Types: Cell Culture Supernates
C Carmona-Fo et al. (2017), Metabolic origins of spatial organization in the tumor microenvironment Proc. Natl. Acad. Sci. U.S.A, 0(0):.
PMID: 28246332 -
Species: Mouse
Sample Types: Tissue Homogenates
WN Jin et al. (2017), Depletion of microglia exacerbates postischemic inflammation and brain injury J. Cereb. Blood Flow Metab., 0(0):271678X176941.
PMID: 28273719 -
Species: Mouse
Sample Types: Peritoneal Fluid
MJ Strowitzki et al. (2017), Pharmacological HIF-inhibition attenuates postoperative adhesion formation Sci Rep, 7(1):13151.
PMID: 29030625 -
Species: Mouse
Sample Types: Cell Culture Supernates
BV Howley et al. (2017), A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Oncogene, 0(0):.
PMID: 29249802 -
Species: Mouse
Sample Types: Cell Lysates
(2016), Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity Proc. Natl. Acad. Sci. U.S.A, 0(0):.
PMID: 27994144 -
Species: Mouse
Sample Types: Cell Culture Supernates
Qi Liu (2016), Evaluation of PLGA containing anti-CTLA4 inhibited endometriosis progression by regulating CD4+CD25+Treg cells in peritoneal fluid of mouse endometriosis model Eur J Pharm Sci, 0(0):.
PMID: 27989857 -
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
Nan Chiang (2016), Novel Resolvin D2 Receptor Axis in Infectious Inflammation J. Immunol, 0(0):.
PMID: 27994074 -
Species: Mouse
Sample Types: Serum
Fernandez-Ruiz R et al. (0), Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells. Nat Commu11(1):5982.
PMID: 33239617
There are no citations that match your criteria.
Customer Reviews (20)
Customer Images
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Verified Customer | Posted 03/14/2021
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Verified Customer | Posted 09/17/2020Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Readout is not a fluorescence so you don't need a special fluorescence reader. If you are doing Western blotting, you can do this easily.
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Verified Customer | Posted 02/12/2020Mouse breast tumor tissues
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Verified Customer | Posted 01/25/2020
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Verified Customer | Posted 01/06/2020Excellent array!!
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Verified Customer | Posted 06/06/2019
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Verified Customer | Posted 03/13/2019
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Verified Customer | Posted 12/05/2018
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Verified Customer | Posted 11/11/2018Excellent specificity and detection for large number of cytokines. Works perfectly with little optimization.
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Verified Customer | Posted 11/05/2018Pancreas lysate. 750ug protein/ membrane
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Verified Customer | Posted 07/18/2018
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Verified Customer | Posted 04/01/2018
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Verified Customer | Posted 03/22/2018
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Verified Customer | Posted 12/07/2017200 uL pooled serum from wild-type C57Bl/6 mice.5 min exposure time
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Verified Customer | Posted 08/17/2017
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Verified Customer | Posted 07/17/2017
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Verified Customer | Posted 05/23/2017
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Verified Customer | Posted 11/28/2016150 uL of mouse serum
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Verified Customer | Posted 09/30/2016This is a great kit. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. I strongly recommend it.
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Verified Customer | Posted 08/26/2016The sample was cell culture supernatant collected from MEF.
There are no reviews that match your criteria.
FAQs
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.