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Proteome Profiler Mouse XL Cytokine Array
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Lysate. Lysates from Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (200 µg lysate, 10 minute exposure). |
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Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate. Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (200 µg lysate, 10 minute exposure). |
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Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate. Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (500 µL of cell culture supernate, 5 minute exposure). |
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, relative expression levels of mouse cytokines and chemokines in samples can be determined using the following procedure:
- Prepare membrane and incubate with prepared sample
- Incubate the membrane with Detection Antibody Cocktail
- Incubate the membrane array with Streptavidin-HRP
- Develop the membrane array with Chemi Reagents 1 and 2
- Expose the membrane array to autoradiography film
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
Other Supplies Required
Reagents
- Aprotinin
- Leupeptin (Catalog #EI002)
- Pepstatin (Catalog #EI003)
- Igepal® CA-630
- Deionized or distilled water
Materials
- Pipettes and pipette tips
- Gloves
- Plastic container with the capacity to hold 50 mL (for washing the arrays)
- Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
- Plastic wrap
- Absorbent lab wipes (KimWipes® or equivalent)
- Paper towels
- X-ray film (Kodak BioMax™ Light-1) or equivalent
- Flat-tipped tweezers
Equipment
- Rocking platform shaker
- Microcentrifuge
- Autoradiography cassette
- Film developer
- Flatbed scanner with transparency adapter capable of transmission mode
- Computer capable of running image analysis software and Microsoft Excel
Other Supplies Required for Cell Lysate Samples
- Phosphate-Buffered Saline (PBS)
- Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
Other Supplies Required for Tissue Lysate Samples
- PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
- Triton™ X-100
Procedure Overview
R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028)
Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.
Place each array membrane in a separate well of the 4-Well Multi-dish.
Incubate for one hour on a rocking platform shaker.
Add 0.5 mL Array Buffer 4 to each sample.
Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.
Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.
Incubate overnight at 2 °C to 8 °C on a rocking platform.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.
Add 30 µL of Detection Antibody Cocktail to 1.5 mL of Array Buffer 4/6 for each array.
Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.
Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.
Incubate for one hour on a rocking platform shaker.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.
Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.
Place the array membrane in the diluted Streptavidin-HRP solution.
Incubate for 30 minutes on a rocking platform shaker.
Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
Place the array membrane on a plastic sheet protector.
Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.
Cover the membrane with the top sheet of the plastic protector.
Incubate for 1 minute.
Blot off excess Chemi Reagent Mix.
Wrap the membrane and sheet protector in plastic wrap.
Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.
Citations for Proteome Profiler Mouse XL Cytokine Array (110)
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Mouse XL Cytokine Array include:
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Species: Mouse
Sample Types: Tissue Homogenates
MS Lee et al. (2023), Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation Oncogene, 20(0):368-380.
PMID: 35784638 -
Species: Mouse
Sample Types: Plasma
A Vorontsova et al. (2023), Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitors Oncogene, 0(0).
PMID: 36646904 -
Species: Mouse
Sample Types: Tissue Homogenates
JS Chae et al. (2023), The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIkappaBs) in Chronic Post-Ischemia Pain (CPIP) Models Pharmaceutics, 15(2).
PMID: 36839876 -
Species: Mouse
Sample Types: Tissue Homogenates
HJ Lee et al. (2022), Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke Neural regeneration research, 17(7):1556-1565.
PMID: 34916441 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Meng et al. (2022), Tumor-derived Jagged1 promotes cancer progression through immune evasion Cell Reports, 38(10):110492.
PMID: 35263601 -
Species: Mouse
Sample Types: Tissue Homogenates
FX Yu et al. (2022), The impact of sensory neuropathy and inflammation on epithelial wound healing in diabetic corneas Progress in retinal and eye research, 0(0):101039.
PMID: 34991965 -
Species: Mouse
Sample Types: Whole Cell
TN Pham et al. (2022), Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity JCI Insight, 0(0).
PMID: 35380995 -
Species: Mouse
Sample Types: Cell Culture Supernants
L Zhong et al. (2022), Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation JCI Insight, 7(7).
PMID: 35393948 -
Species: Mouse
Sample Types: Cell Culture Supernates
S Ryu et al. (2022), Loss of RNA binding protein HuD facilitates the production of the senescence-associated secretory phenotype Cell Death Disease, 2022;13(4):329.
PMID: 35411051 -
Species: Mouse
Sample Types: Plasma
K Sundaram et al. (2022), Garlic exosome-like nanoparticles reverse high-fat diet induced obesity via the gut/brain axis Theranostics, 12(3):1220-1246.
PMID: 35154484 -
Species: Mouse
Sample Types: Plasma
A Kumar et al. (2022), Ginger nanoparticles mediated induction of Foxa2 prevents high-fat diet-induced insulin resistance Theranostics, 12(3):1388-1403.
PMID: 35154496 -
Species: Mouse
Sample Types: Cell Lysates
BG Hunt et al. (2022), RON (MST1R) and HGFL (MST1) Co-Overexpression Supports Breast Tumorigenesis through Autocrine and Paracrine Cellular Crosstalk Cancers, 14(10).
PMID: 35626096 -
Species: Mouse
Sample Types: Protein Lysates
HC Pan et al. (2022), Essential Roles of the Histone Demethylase KDM4C in Renal Development and Acute Kidney Injury International Journal of Molecular Sciences, 23(16).
PMID: 36012577 -
Species: Mouse
Sample Types: Tissue Lysates
HY Kim et al. (2022), Recombinant FGF21 Attenuates Polychlorinated Biphenyl-Induced NAFLD/NASH by Modulating Hepatic Lipocalin-2 Expression International Journal of Molecular Sciences, 23(16).
PMID: 36012166 -
VC Sanchez et al. (2022), Host CLIC4 expression in the tumor microenvironment is essential for breast cancer metastatic competence PloS Genetics, 18(6):e1010271.
PMID: 35727842 -
Species: Mouse
Sample Types: Tissue Homogenates
S Räuber et al. (2022), Modulation of Rxralpha Expression in Mononuclear Phagocytes Impacts on Cardiac Remodeling after Ischemia-Reperfusion Injury Biomedicines, 10(6).
PMID: 35740296 -
Species: Mouse
Sample Types: Tissue Homogenates
N Tisch et al. (2022), Caspase-8 in endothelial cells maintains gut homeostasis and prevents small bowel inflammation in mice Embo Molecular Medicine, 0(0):e14121.
PMID: 35491615 -
Species: Mouse
Sample Types: Cell Culture Supernates
HC West et al. (2022), Loss of T�cell tolerance in the skin following immunopathology is linked to failed restoration of the dermal niche by recruited macrophages Cell Reports, 39(7):110819.
PMID: 35584681 -
Species: Mouse
Sample Types: Serum
M Balogh et al. (2022), Sensory neuron dysfunction in orthotopic mouse models of colon cancer Journal of Neuroinflammation, 19(1):204.
PMID: 35962398 -
Species: Mouse
Sample Types: Cell Culture Supernates
AE Pottash et al. (2022), Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy Non-coding RNA, 8(5).
PMID: 36287123 -
Species: Mouse
Sample Types: Tissue Homogenates
R Komori et al. (2022), Regulation of Inflammation-Related Genes through Fosl1 Suppression in a Levetiracetam-Treated Pilocarpine-Induced Status Epilepticus Mouse Model International Journal of Molecular Sciences, 23(14).
PMID: 35886955 -
Species: Mouse
Sample Types: Tissue Homogenates
T Matsuo et al. (2022), Levetiracetam Suppresses the Infiltration of Neutrophils and Monocytes and Downregulates Many Inflammatory Cytokines during Epileptogenesis in Pilocarpine-Induced Status Epilepticus Mice International Journal of Molecular Sciences, 23(14).
PMID: 35887020 -
Species: Mouse
Sample Types: Plasma
H Blanton et al. (2022), Cannabidiol and Beta-Caryophyllene in Combination: A Therapeutic Functional Interaction International Journal of Molecular Sciences, 23(24).
PMID: 36555111 -
Species: Mouse
Sample Types: Serum
SH Seo et al. (2022), Metabolic improvement and liver regeneration by inhibiting CXXC5 function for non-alcoholic steatohepatitis treatment Experimental Molecular Medicine, 2022;0(0).
PMID: 36114279 -
Species: Mouse
Sample Types: Whole Cells
M Jung et al. (2022), RNA binding protein HuD mediates the crosstalk between beta cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression Cell Death Disease, 2022;13(12):1019.
PMID: 36470872 -
Species: Mouse
Sample Types: Cell Culture Supernates
HG Roweth et al. (2022), Pro-inflammatory megakaryocyte gene expression in murine models of breast cancer Science Advances, 8(41):eabo5224.
PMID: 36223471 -
Species: Mouse
Sample Types: Cell Culture Supernates
T Huu Hoang et al. (2022), Cancer cells produce liver metastasis via gap formation in sinusoidal endothelial cells through proinflammatory paracrine mechanisms Science Advances, 8(39):eabo5525.
PMID: 36170363 -
Species: Mouse
Sample Types: Cell Lysates
D Zimmerli et al. (2022), MYC promotes immune-suppression in triple-negative breast cancer via inhibition of interferon signaling Nature Communications, 13(1):6579.
PMID: 36323660 -
Species: Mouse
Sample Types: Serum
JY Hou et al. (2021), Integration of transcriptomics and system pharmacology to reveal the therapeutic mechanism underlying Qingfei Xiaoyan Wan to treat allergic asthma Journal of ethnopharmacology, 278(0):114302.
PMID: 34090911 -
Species: Mouse
Sample Types: Cell Culture Supernates
E Yeini et al. (2021), P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature Communications, 12(1):1912.
PMID: 33771989 -
Species: Mouse
Sample Types: Peritoneal Fluid
T Ito et al. (2021), Cell barrier function of resident peritoneal macrophages in post-operative adhesions Nature Communications, 12(1):2232.
PMID: 33854051 -
Species: Mouse
Sample Types: Cell Culture Supernates
X Sun et al. (2021), Preventing tumor progression to the bone by induced tumor-suppressing MSCs Theranostics, 11(11):5143-5159.
PMID: 33859739 -
Species: Mouse
Sample Types: Cell Lysates
K Li et al. (2021), CD8+ T cell immunity blocks the metastasis of carcinogen-exposed breast cancer Science Advances, 7(25).
PMID: 34144976 -
Species: Mouse
Sample Types: Tissue Homogenates
D Paramos-de et al. (2021), Targeting senescent cells improves functional recovery after spinal cord injury Cell Reports, 36(1):109334.
PMID: 34233184 -
Species: Mouse
Sample Types: Tissue Homogenates
MJ Strowitzki et al. (2021), Inhibition of HIF-prolyl hydroxylases improves healing of intestinal anastomoses JCI Insight, 0(0).
PMID: 33784253 -
Species: Mouse
Sample Types: Plasma
R Pandey et al. (2021), JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific Reports, 11(1):7372.
PMID: 33795715 -
Species: Mouse
Sample Types: Tissue Homogenates
Q Li et al. (2021), Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36 Brain, Behavior, and Immunity, 94(0):437-457.
PMID: 33588074 -
Species: Mouse
Sample Types: Tissue Homogenates
CS Thomson et al. (2021), WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth Oncogene, 40(24):4229-4241.
PMID: 34079083 -
Species: Mouse
Sample Types: Serum
T Masaki et al. (2021), GIP_HUMAN[22-51] is a new proatherogenic peptide identified by native plasma peptidomics Scientific Reports, 11(1):14470.
PMID: 34262109 -
Species: Mouse
Sample Types: Cell Culture Supernates
LM Snyder et al. (2021), Induction of IL-12p40 and type 1 immunity by Toxoplasma gondii in the absence of the TLR-MyD88 signaling cascade PloS Pathogens, 17(10):e1009970.
PMID: 34597344 -
Species: Mouse
Sample Types: Cell Culture Supernates
D Chanda et al. (2021), Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells Elife, 10(0).
PMID: 34528872 -
Species: Mouse
Sample Types: Plasma
R Samain et al. (2021), Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 33482394 -
Species: Mouse
Sample Types: Serum
Y Feng et al. (2021), Mechanical Loading-Driven Tumor Suppression Is Mediated by Lrp5-Dependent and Independent Mechanisms Cancers, 13(2).
PMID: 33450808 -
Species: Mouse
Sample Types: Cell Culture Supernates
N Ogawa et al. (2021), Trimebutine suppresses Toll-like receptor 2/4/7/8/9 signaling pathways in macrophages Archives of biochemistry and biophysics, 711(0):109029.
PMID: 34517011 -
Species: Mouse
Sample Types: Plasma
F Yao et al. (2021), A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 12(1):7333.
PMID: 34921145 -
Species: Mouse
Sample Types: Cell Culture Supernates
HL Desu et al. (2021), TNFR2 Signaling Regulates the Immunomodulatory Function of Oligodendrocyte Precursor Cells Cells, 10(7).
PMID: 34359956 -
Species: Mouse
Sample Types: Serum
P Mamidi et al. (2021), MK2a inhibitor CMPD1 abrogates chikungunya virus infection by modulating actin remodeling pathway Oncogene, 17(11):e1009667.
PMID: 34780576 -
Species: Mouse
Sample Types: Plasma
A Kumar et al. (2021), High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Nature Communications, 12(1):213.
PMID: 33431899 -
Species: Mouse
Sample Types: Tissue Homogenates
JD Bryant et al. (2021), Neuroimmune mechanisms of cognitive impairment in a mouse model of Gulf War illness Brain, Behavior, and Immunity, 0(0).
PMID: 34333111 -
Species: Mouse
Sample Types: Cell Culture Supernates
S Xiao et al. (2021), Innate immune regulates cutaneous sensory IL-13 receptor alpha 2 to promote atopic dermatitis Brain, Behavior, and Immunity, 98(0):28-39.
PMID: 34391816 -
Species: Mouse
Sample Types: Cell Culture Supernates
M Mizuno et al. (2021), Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell Interaction Cancers, 13(16).
PMID: 34439392 -
Species: Mouse
Sample Types: Protein Lysates
P Jiranugrom et al. (2020), NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet Genes (Basel), 11(5).
PMID: 32438638 -
Species: Mouse
Sample Types: Cell Culture Supernates
HL Mercer et al. (2020), Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity PLoS Pathog., 16(5):e1008572.
PMID: 32413093 -
Species: Mouse
Sample Types: Serum
Y Cheng et al. (2020), Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages PLoS Pathog., 16(5):e1008569.
PMID: 32463840 -
Species: Mouse
Sample Types: Serum
D Ren et al. (2020), Sestrin2 modulates cardiac inflammatory response through maintaining redox homeostasis during ischemia and reperfusion Redox Biol, 34(0):101556.
PMID: 32447260 -
Species: Mouse
Sample Types: Cell Lysates
AM Decker et al. (2020), Adrenergic Blockade Promotes Maintenance of Dormancy in Prostate Cancer Through Upregulation of GAS6 Transl Oncol, 13(7):100781.
PMID: 32361123 -
Species: Mouse
Sample Types: Cell Culture Supernates
AA Hartman et al. (2020), YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells Stem Cell Reports, 14(4):730-743.
PMID: 32243844 -
Species: Mouse
Sample Types: Tissue Homogenates
W He et al. (2020), High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation Nat Commun, 11(1):1732.
PMID: 32265505 -
Species: Mouse
Sample Types: Tissue Homogenates
HY Lin et al. (2020), SUMO E3 ligase PIAS1 is a potential biomarker indicating stress susceptibility Psychoneuroendocrinology, 120(0):104800.
PMID: 32688147 -
Species: Mouse
Sample Types: Tissue Homogenates
Y Liu et al. (2020), Triterpenoids Extracted From Antrodia cinnamomea Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice via Suppression Inflammatory Response Front Microbiol, 11(0):1113.
PMID: 32719658 -
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
K Hirashima et al. (2020), The mechanism of Tyk2 deficiency-induced immunosuppression in mice involves robust IL-10 production in macrophages Cytokine, 130(0):155077.
PMID: 32208335 -
Species: Mouse
Sample Types: Cell Culture Lysates
F Demirciogl et al. (2020), Cancer associated fibroblast FAK regulates malignant cell metabolism Nat Commun, 11(1):1290.
PMID: 32157087 -
Species: Mouse
Sample Types: Tissue Lysates
X Zhou et al. (2020), Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2 Nat. Neurosci., 23(3):337-350.
PMID: 32112058 -
Species: Mouse
Sample Types: Whole Cells
CC Hu et al. (2020), Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects Int J Mol Sci, 21(15).
PMID: 32756396 -
Species: Mouse
Sample Types: Tissue Homogenates
MT Hoffman et al. (2020), The Gustatory Sensory G-Protein GNAT3 Suppresses Pancreatic Cancer Progression in Mice Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32882403 -
Species: Mouse
Sample Types: Serum
MB Preda et al. (2020), Evidence of mesenchymal stromal cell adaptation to local microenvironment following subcutaneous transplantation J. Cell. Mol. Med., 0(0).
PMID: 32785979 -
Species: Mouse
Sample Types: Plasma
S Marquard et al. (2020), Yes-associated protein (YAP) induces a secretome phenotype and transcriptionally regulates plasminogen activator Inhibitor-1 (PAI-1) expression in hepatocarcinogenesis Cell Commun Signal, 18(1):166.
PMID: 33097058 -
Species: Mouse
Sample Types: Tissue Homogenates
YJ Park et al. (2020), Bisphenol A exposure increases epididymal susceptibility to infection in mice Ecotoxicol Environ Saf, 208(0):111476.
PMID: 33091778 -
Species: Mouse
Sample Types: Serum
HJ Wang et al. (2020), Adaptive thermogenesis enhances the life-threatening response to heat in mice with an Ryr1 mutation Nat Commun, 11(1):5099.
PMID: 33037202 -
Species: Mouse
Sample Types: Cell Lysates
JS Russell et al. (2020), Dietary &alpha-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection Nutrients, 12(9).
PMID: 32887376 -
Species: Mouse
Sample Types: Plasma
WN Jin et al. (2020), Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition Nature Neuroscience, 0(0).
PMID: 33257875 -
Species: Mouse
Sample Types: Cell Culture Supernates
A Banisadr et al. (2020), EGFRvIII uses intrinsic and extrinsic mechanisms to reduce glioma adhesion and increase migration J Cell Sci, 0(0).
PMID: 33243756 -
Species: Mouse
Sample Types: Cell Culture Supernates
K Lim et al. (2020), In situ neutrophil efferocytosis shapes T cell immunity to influenza infection Nat. Immunol., 0(0).
PMID: 32747818 -
Species: Mouse
Sample Types: Cell Culture Supernates
Y Guan et al. (2020), Senescence-activated enhancer landscape orchestrates the senescence-associated secretory phenotype in murine fibroblasts Nucleic Acids Res, 0(0).
PMID: 33045748 -
Species: Mouse
Sample Types: Serum
R Fernandez- et al. (2020), Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells Nat Commun, 11(1):5982.
PMID: 33239617 -
Species: Mouse
Sample Types: Cell Lysates
B Lee et al. (2020), An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1 Autophagy, 0(0):1-18.
PMID: 32579471 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Huang et al. (2020), Hyperactivity of Innate Immunity Triggers Pain via TLR2-IL-33-Mediated Neuroimmune Crosstalk Cell Rep, 33(1):108233.
PMID: 33027646 -
Species: Mouse
Sample Types: Cell Culture Supernates
CC Hu et al. (2020), Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects Int J Mol Sci, 21(15).
PMID: 32756396 -
Species: Mouse
Sample Types: Serum
MB Preda et al. (2020), Evidence of mesenchymal stromal cell adaptation to local microenvironment following subcutaneous transplantation J. Cell. Mol. Med., 0(0).
PMID: 32785979 -
Species: Mouse
Sample Types: Tissue Homogenates
K Lim et al. (2020), In situ neutrophil efferocytosis shapes T cell immunity to influenza infection Nat. Immunol., 0(0).
PMID: 32747818 -
Species: Mouse
Sample Types: Cell Culture Supernates
Y Cho et al. (2020), Intramembrane proteolysis of an extracellular serine protease, epithin/PRSS14, enables its intracellular nuclear function BMC Biol., 18(1):60.
PMID: 32493324 -
Species: Mouse
Sample Types: Plasma
J Liu et al. (2019), Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output EBioMedicine, 0(0).
PMID: 30827929 -
Species: Mouse
Sample Types: Cell Lysates
W Wang et al. (2019), Prostate cancer promotes a vicious cycle of bone metastasis progression through inducing osteocytes to secrete GDF15 that stimulates prostate cancer growth and invasion Oncogene, 0(0).
PMID: 30755731 -
Species: Mouse
Sample Types: Tissue Homogenates
M Malehmir et al. (2019), Platelet GPIb? is a mediator and potential interventional target for NASH and subsequent liver cancer Nat. Med., 25(4):641-655.
PMID: 30936549 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Schyns et al. (2019), Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung Nat Commun, 10(1):3964.
PMID: 31481690 -
Species: Mouse
Sample Types: Matrix Bound Nanovesicles
GS Hussey et al. (2019), Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway J Immunol Regen Med, 3(0):26-35.
PMID: 31656879 -
Species: Mouse
Sample Types: Tissue Homogenates
F Hempel et al. (2019), Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice Cells, 8(10).
PMID: 31591328 -
Species: Mouse
Sample Types: Cell Culture Supernates
A El-Kenawi et al. (2019), Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer Br. J. Cancer, 121(7):556-566.
PMID: 31417189 -
Species: Mouse
Sample Types: Whole Cells
K Haruwaka et al. (2019), Dual microglia effects on blood brain barrier permeability induced by systemic inflammation Nat Commun, 10(1):5816.
PMID: 31862977 -
Species: Mouse
Sample Types: Cell Culture Supernates
A Gordon-Wee et al. (2019), Tumour-Derived Laminin alpha5 (LAMA5) Promotes Colorectal Liver Metastasis Growth, Branching Angiogenesis and Notch Pathway Inhibition Cancers (Basel), 11(5).
PMID: 31064120 -
Species: Mouse
Sample Types: Serum
P Chang et al. (2018), Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine Cell Death Dis, 9(2):154.
PMID: 29402989 -
Species: Mouse
Sample Types: Tissue Homogenates
A Calcinotto et al. (2018), IL-23 secreted by myeloid cells drives castration-resistant prostate cancer Nature, 0(0).
PMID: 29950727 -
Species: Mouse
Sample Types: Cell Culture Supernates
KB Gama et al. (2018), Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation Stem Cells Int, 2018(0):8179013.
PMID: 29535781 -
Species: Mouse
Sample Types: Tissue Homogenates
RA Brady et al. (2018), Comparison of the immune response during acute and chronic Staphylococcus aureus infection PLoS ONE, 13(3):e0195342.
PMID: 29596507 -
Species: Mouse
Sample Types: Cell Culture Supernates
X Sun et al. (2018), Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulatingadipose stromal interleukin 6 production in mice J. Biol. Chem., 0(0).
PMID: 29298894 -
Species: Mouse
Sample Types: Cell Lysates
HR Kim et al. (2018), T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells Nat Commun, 9(1):3630.
PMID: 30194420 -
Species: Mouse
Sample Types: Plasma
T Szatmári et al. (2018), Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood Int. J. Radiat. Biol., 0(0):1-31.
PMID: 29533121 -
Species: Mouse
Sample Types: Cell Lysates
T Kobori et al. (2018), Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis Front Immunol, 9(0):334.
PMID: 29559970 -
Species: Mouse
Sample Types: Peritoneal Fluid
MJ Strowitzki et al. (2017), Pharmacological HIF-inhibition attenuates postoperative adhesion formation Sci Rep, 7(1):13151.
PMID: 29030625 -
Species: Mouse
Sample Types: Tissue Homogenates
WN Jin et al. (2017), Depletion of microglia exacerbates postischemic inflammation and brain injury J. Cereb. Blood Flow Metab., 0(0):271678X176941.
PMID: 28273719 -
Species: Mouse
Sample Types: Cell Culture Supernates
C Carmona-Fo et al. (2017), Metabolic origins of spatial organization in the tumor microenvironment Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28246332 -
Species: Mouse
Sample Types: Cell Culture Supernates
BV Howley et al. (2017), A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Oncogene, 0(0).
PMID: 29249802 -
Species: Mouse
Sample Types: Cell Culture Supernates
D Ribet et al. (2017), Promyelocytic Leukemia Protein (PML) Controls Listeria monocytogenes Infection MBio, 8(1).
PMID: 28074026 -
Species: Mouse
Sample Types: Cell Lysates
K Murakami et al. (2017), A Jak1/2 inhibitor, baricitinib, inhibits osteoclastogenesis by suppressing RANKL expression in osteoblasts in vitro PLoS ONE, 12(7):e0181126.
PMID: 28708884 -
Species: Mouse
Sample Types: Tissue Homogenates
Á Sánchez et al. (2017), Map3k8 controls granulocyte colony-stimulating factor production and neutrophil precursor proliferation in lipopolysaccharide-induced emergency granulopoiesis Sci Rep, 7(1):5010.
PMID: 28694430 -
Species: Mouse
Sample Types: Tissue Homogenates
X Lu et al. (2017), Effective combinatorial immunotherapy for castration-resistant prostate cancer Nature, 543(7647):728-732.
PMID: 28321130 -
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
Nan Chiang (2016), Novel Resolvin D2 Receptor Axis in Infectious Inflammation J. Immunol, 0(0).
PMID: 27994074 -
Species: Mouse
Sample Types: Cell Culture Supernates
Qi Liu (2016), Evaluation of PLGA containing anti-CTLA4 inhibited endometriosis progression by regulating CD4+CD25+Treg cells in peritoneal fluid of mouse endometriosis model Eur J Pharm Sci, 0(0).
PMID: 27989857 -
Species: Mouse
Sample Types: Cell Lysates
(2016), Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 27994144 -
Species: Mouse
Sample Types: Serum
Fernandez-Ruiz R et al. (0), Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells. Nat Commu11(1):5982.
PMID: 33239617
There are no citations that match your criteria.
Customer Reviews for Proteome Profiler Mouse XL Cytokine Array (30)
4.9 out of 5
30 customer ratings
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Verified Customer | Posted 05/06/2023This product works very well
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Verified Customer | Posted 10/16/2022Conditioned medium of macrophages (36h) isolated from metastatic lungs.
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Verified Customer | Posted 08/28/2022This kit is really great!
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Verified Customer | Posted 08/07/2022Secretome of 4T1 murine breast cancer cells.
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Verified Customer | Posted 08/07/2022Cytokine array of the secretome of MLE12 cells.
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Verified Customer | Posted 07/11/2022
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Verified Customer | Posted 12/02/2021
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Verified Customer | Posted 10/21/2021
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Verified Customer | Posted 09/16/2021
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Verified Customer | Posted 08/10/2021We used the array kit for profiling inflammatory cytokines in plasma samples from mice. I trust that it works very well.
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Verified Customer | Posted 03/14/2021
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Verified Customer | Posted 09/17/2020Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Readout is not a fluorescence so you don't need a special fluorescence reader. If you are doing Western blotting, you can do this easily.
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Verified Customer | Posted 02/12/2020Mouse breast tumor tissues
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Verified Customer | Posted 01/25/2020
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Verified Customer | Posted 01/06/2020Excellent array!!
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Verified Customer | Posted 06/06/2019
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Verified Customer | Posted 03/13/2019
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Verified Customer | Posted 12/05/2018
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Verified Customer | Posted 11/11/2018Excellent specificity and detection for large number of cytokines. Works perfectly with little optimization.
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Verified Customer | Posted 11/05/2018Pancreas lysate. 750ug protein/ membrane
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Verified Customer | Posted 07/18/2018
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Verified Customer | Posted 04/01/2018
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Verified Customer | Posted 03/22/2018
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Verified Customer | Posted 12/07/2017200 uL pooled serum from wild-type C57Bl/6 mice.5 min exposure time
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Verified Customer | Posted 08/17/2017
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Verified Customer | Posted 07/17/2017
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Verified Customer | Posted 05/23/2017
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Verified Customer | Posted 11/28/2016150 uL of mouse serum
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Verified Customer | Posted 09/30/2016This is a great kit. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. I strongly recommend it.
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Verified Customer | Posted 08/26/2016The sample was cell culture supernatant collected from MEF.
There are no reviews that match your criteria.
FAQs for Proteome Profiler Mouse XL Cytokine Array
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
