Mouse M-CSF DuoSet ELISA

Name: Mouse M-CSF DuoSet ELISA
Brand: R&D Systems
SKU: DY416
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY416

Citations (8)

(1)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	15.6 - 1,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse M-CSF DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Mouse M-CSF DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse M-CSF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Mouse M-CSF DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse M-CSF DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Mouse M-CSF DuoSet ELISA

	Mouse M-CSF ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Item	Value
Long Name	Macrophage Colony Stimulating Factor
Entrez Gene IDs	1435 (Human); 12977 (Mouse)
Alternate Names	CSF-1, CSF1, Lanimostim, M-CSF, MCSF, MCSFlanimostim, MGC31930, colony stimulating factor 1 (macrophage), macrophage colony stimulating factor, macrophage colony-stimulating factor 1

Citations for Mouse M-CSF DuoSet ELISA (8)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse M-CSF DuoSet ELISA include:

Species: Human
Sample Types: Tissue Homogenates

B Khaleel et al. (2022), Effect of Chemotherapy Cytarabine and Acute Myeloid Leukemia on the Development of Spermatogenesis at the Adult Age of Immature Treated Mice International Journal of Molecular Sciences, 23(7).
PMID: 35409373
Species: Mouse
Sample Types: Cell Culture Supernates

LE Hand et al. (2019), The circadian regulator Bmal1 in joint mesenchymal cells regulates both joint development and inflammatory arthritis Arthritis Res. Ther., 21(1):5.
PMID: 30612576
Species: Mouse
Sample Types: Cell Culture Supernates

AW Pountain et al. (2019), Genomic instability at the locus of sterol C24-methyltransferase promotes amphotericin B resistance in Leishmania parasites PLoS Negl Trop Dis, 13(2):e0007052.
PMID: 30716073
Species: Mouse
Sample Types: Tissue Homogenates

(2016), M-CSF Mediates Host Defense during Bacterial Pneumonia by Promoting the Survival of Lung and Liver Mononuclear Phagocytes J Immunol, 0(0).
PMID: 27183631
Species: Mouse
Sample Types: Tissue Homogenates

Wu K et al. (2015), TREM-2 promotes macrophage survival and lung disease after respiratory viral infection. J Exp Med, 212(5):681-97.
PMID: 25897174
Species: Mouse
Sample Types: Cell Lysates

Escamilla J et al. (2015), CSF1 receptor targeting in prostate cancer reverses macrophage-mediated resistance to androgen blockade therapy. Cancer Res, 75(6):950-62.
PMID: 25736687
Species: Mouse
Sample Types: BALF

Serezani CH et al. (2012), Macrophage Dectin-1 Expression Is Controlled by Leukotriene B4 via a GM-CSF/PU.1 Axis. J. Immunol., 189(2):906-15.
PMID: 22696442
Species: Mouse
Sample Types: Cell Culture Supernates

Chuquimia OD et al. (2012), The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems. PLoS ONE, 7(2):e32125.
PMID: 22393384

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Mouse M-CSF DuoSet ELISA, 15 Plate

Name: Anonymous

Sample Tested: Mouse ear fibroblast

Species: Mouse

Verified Customer | Posted 10/26/2015

Specificity: SpecificSensitivity: SensitiveBuffer: 1% BSA in PBS, pH 7.2 - 7.4,Dilution: 1/180

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