Mouse IL-3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M3000
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
2.5 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse IL-3 Quantikine ELISA Kit
The Quantikine Mouse IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-3 in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse IL-3 and antibodies raised against recombinant mouse IL-3. This Immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-3 showed dose curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 33 | 119 | 396 | 32 | 120 | 400 |
| Standard Deviation | 1.7 | 8.3 | 22 | 2.5 | 5.5 | 18 |
| CV% | 5.2 | 7.0 | 5.6 | 7.8 | 4.6 | 4.5 |
Recovery for Mouse IL-3 Quantikine ELISA Kit
The recovery of mouse IL-3 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 104 | 91-114 |
| Serum (n=5) | 96 | 90-106 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-3 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-3 Quantikine ELISA Kit
Mouse IL-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-3
Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.
Long Name
Interleukin 3
Alternate Names
IL3, MCGF, MULTI-CSF
Gene Symbol
IL3
Additional IL-3 Products
Product Documents for Mouse IL-3 Quantikine ELISA Kit
Product Specific Notices for Mouse IL-3 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.